ribA

ribA
168

GTP cyclohydrolase II/ 3,4-dihydroxy-2-butanone 4-phosphate synthase

Locus
BSU_23260
Molecular weight
43.96 kDa
Isoelectric point
5.58
Protein length
398 aaSequenceBlast
Gene length
1197 bpSequenceBlast
Function
riboflavin biosynthesis
Product
GTP cyclohydrolase II/ 3,4-dihydroxy-2-butanone 4-phosphate synthase
Essential
no
E.C.
3.5.4.25
Synonyms
ribA, ribBA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0807 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,428,389 → 2,429,585
The protein
Catalyzed reaction/ biological activity
D-ribulose 5-phosphate --> (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H+ (according to UniProt)
GTP + 3 H2O --> 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate + formate + 2 H+ (according to UniProt)
Protein family
N-terminal part: DHBP synthase family (single member, according to UniProt)
C-terminal part: GTP cyclohydrolase II family (single member, according to UniProt)
Structure
2BZ0 (PDB) (from E. coli, 54% identity, 69% similarity to  the C-terminal domain) PubMed
P17620 (AlphaFold)
Localization
cytoplasm PubMed
Expression and Regulation
Operons
Genes
ribD-ribE-ribA-ribH-ribT
Description
PubMed
Regulation
expressed in the absence of FMN (FMN-box) PubMed
binding of RibR to the FMN-box riboswitch can enforce expression even in the presence of FMN PubMed
the FMN-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
FMN-box: RNA switch, via FMN-box in the presence of FMN or FMNH2, this is counter-acted upon binding of RibR, in FMN-box
RibR: antitermination, PubMed, in ribR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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ribD→ribT

2025-04-16 00:23:22

Jstuelk

124

3D660447137821317811200D29A5FC04A41E938E

05C8A4E892E7DB3D953FE72E8765638644759326

Biological materials
Mutant
BKE23260 (ΔribA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGAAACATGCAAATCTTCC,  downstream forward: _UP4_TAATCACAAATATCACAAAA
BKK23260 (ΔribA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGAAACATGCAAATCTTCC,  downstream forward: _UP4_TAATCACAAATATCACAAAA
References
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Time of last update: 2025-04-16 02:37:19

Author of last update: Jstuelk