sinR
168

transcriptional regulator (Xre family) of post-exponential-phase responses genes

Locus

BSU_24610

Molecular weight

12.85 kDa

Isoelectric point

7.18

Protein length

111 aaSequenceBlast

Gene length

336 bpSequenceBlast

Function

control of biofilm formation

Product

transcriptional regulator (Xre family)

Essential

Synonyms

sinR, sin, flaD

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1396 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

2,552,653 2,552,988

Phenotypes of a mutant

the mutation suppresses the galactose toxicity to a galE mutant PubMed

Additional information

the sinR gene is often inactivated during adaptation to Arabidopsis thaliana PubMed

improved colonization on plant roots (inactivating mutations appear during growth on roots) PubMed

The protein

Catalyzed reaction/ biological activity

transcription regulator of biofilm genes, acts as a true repressor of the ''tapA-sipW-tasA'' operon and as an anti-activator (prevents binding of the activator protein RemA) of the ''epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO'' operon PubMed

Protein family

Xre family

Domains

DNA-binding N-terminal domain (aa 1-69) PubMed

SinI-binding C-terminal domain (aa 74-111) PubMed

HTH cro/C1-type domain (aa 6-61) (according to UniProt)

Sin domain (aa 65-103) (according to UniProt)

Structure

2YAL (PDB) (C-terminal domain, aa 74-111) PubMed

3QQ6 (PDB) (N-terminal domain, aa 1-69) PubMed

1B0N (PDB) (complex SinR-SinI) PubMed

P06533 (AlphaFold)

Effectors of protein activity

SinI and SlrA are antagonists to SinR PubMed

SlrR is an antagonist of SinR, but also acts as cofactor for SinR resulting in binding to sites within the fliE and fliI open reading frames and to premature stopp of transcription elongation PubMed

Paralogous protein(s)

SlrR

Expression and Regulation

Operons

Genes

sinI-sinR

Description

PubMed

Regulation

repressed during exponential growth (ScoC) PubMed

Regulatory mechanism

Spo0A: activation, in a portion of cells PubMed, in spo0A regulon