epsA

epsA
168

extracellular polysaccharide synthesis, putative transmembrane modulator of EpsB activity, might activate EpsB autophosphorylation and substrate phosphorylation

Locus
BSU_34370
Molecular weight
25.75 kDa
Isoelectric point
6.07
Protein length
234 aaSequenceBlast
Gene length
705 bpSequenceBlast
Function
biofilm formation
Product
unknown
Essential
no
Synonyms
epsA, yveK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3944 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,529,151 → 3,529,855
The protein
Protein family
CapA family (with CapA and TkmA, according to UniProt)
CpsC/CapA family (with TkmA, according to UniProt)
Structure
P71050 (AlphaFold)
Paralogous protein(s)
TkmA
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO
Description
PubMed
Regulation
repressed by SinR PubMed
Regulatory mechanism
RemA: activation, PubMed, in remA regulon
AbrB: repression, PubMed, in abrB regulon
SinR: anti-activation, (prevents binding of RemA) PubMed, in sinR regulon
EAR riboswitch: processive antitermination, in EAR riboswitch
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
induction by sequestration of SinR by SinI or SlrA PubMed
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epsA→epsO

2025-04-02 11:08:39

ghost

150

972f8e32dbf02ccc05ec1b86b52f3e7c715bf4da

DAC70A15EA1F43CB18727990D26DF72F59759165

Biological materials
Mutant
MGNA-A073 (yveK::erm), available at the NBRP B. subtilis, Japan
GP1517 (aphA3) PubMed, available in Jörg Stülke's lab
GP1519 (''epsA-epsB'', aphA3) PubMed, available in Jörg Stülke's lab
GP1567 ''epsA::aphA3 tkmA''::spc PubMed, available in Jörg Stülke's lab
BKE34370 (ΔepsA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTATTCATAGCCTTCAG,  downstream forward: _UP4_AAACATTTCGGGGAGTGAAG
BKK34370 (ΔepsA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTATTCATAGCCTTCAG,  downstream forward: _UP4_AAACATTTCGGGGAGTGAAG
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH) PubMed, available in Jörg Stülke's lab
FLAG-tag construct
GP1526 epsA-FLAG 3x spc (based on pGP1331) PubMed, available in Jörg Stülke's lab
Antibody
**
GFP fusion
GP1569 epsA-gfp (spc), available in Jörg Stülke's lab
Labs working on this gene/protein
Richard Losick, Harvard Univ., Cambridge, USA homepage
References
Reviews
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Original Publications
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F6C4634BF871D9A01E27014FFED7527C54BF560D

Page visits: 8628

Time of last update: 2025-04-04 09:55:37

Author of last update: Jstuelk