addB
168

ATP-dependent deoxyribonuclease (subunit B), required for efficient survival and replication restart after replication-transcription conflicts, responsible for end resection during dsDNA break repair

Locus

BSU_10620

Molecular weight

134.41 kDa

Isoelectric point

5.39

Protein length

1166 aaSequenceBlast

Gene length

3501 bpSequenceBlast

Function

DNA repair/ recombination

Product

ATP-dependent deoxyribonuclease (subunit B))

Essential

Synonyms

addB

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

Information processing, Genetics, DNA repair/ recombination

Information processing, Genetics, DNA repair/ recombination, Other proteins

Information processing, Genetics, Genetic competence

Lifestyles, Exponential and early post-exponential lifestyles, Genetic competence

List of homologs in different organisms, belongs to COG3857 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, ComK regulon

Gene

Coordinates

1,136,320 1,139,820

Phenotypes of a mutant

reduced survival after mitomycin treatment PubMed

''recO addA-addB'' double mutants are extremely sensitive against DNA damaging agents PubMed

The protein

Catalyzed reaction/ biological activity

the enzyme is functional as a heterodimer of the AddA and AddB subunits, that it is a rapid and processive DNA helicase, and that it catalyses DNA unwinding using one single-stranded DNA motor of 3'5' polarity located in the AddA subunit PubMed

the AddB subunit contains a second putative ATP-binding pocket, but this does not contribute to the observed helicase activity and may instead be involved in the recognition of recombination hotspot sequences PubMed

ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

helicase family (according to UniProt)

Domains

UvrD-like helicase ATP-binding domain(aa 1-278) (according to UniProt)

UvrD-like helicase C-terminal domain (aa 281-586) (according to UniProt)

Cofactors

Fe-S cluster PubMed

Structure

3U4Q (PDB) (the AddA-AddB-DNA complex) PubMed

P23477 (AlphaFold)

Expression and Regulation