efeB

efeB
168

elemental iron uptake system, A-type dye-decolorizing peroxidase, converts ferrous iron (Fe(II) to ferric iron (FeIII)) for uptake by EfeO-EfeU, peroxide detoxification under microaerobic conditions

Locus
BSU_38260
Molecular weight
45.53 kDa
Isoelectric point
8.64
Protein length
416 aaSequenceBlast
Gene length
1251 bpSequenceBlast
Function
ferrous iron conversion
Product
heme peroxidase in elemental iron uptake
Essential
no
E.C.
1.11.1.-
Synonyms
efeB, efeN, ipa-29d, ywbN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2837 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,926,682  3,927,932
Phenotypes of a mutant
poor growth with Fe(II) under microaerobic conditions PubMed
The protein
Catalyzed reaction/ biological activity
oxidizes ferrous iron to ferric iron for uptake by EfeO-EfeU PubMed
eliminates reactive oxygen species that accumulate in the presence of ferrous iron PubMed
peroxide detoxification under microaerobic conditions PubMed
Protein family
DyP-type peroxidase family (single member, according to UniProt)
Cofactors
protoheme IX PubMed
Structure
6KMN (PDB) PubMed
7PKX (PDB) PubMed
7E5Q (PDB) (aa 56 ... 416) PubMed
P39597 (AlphaFold)
Effectors of protein activity
activity is increased in the presence of EfeO due to the displacement of ferric iron PubMed
Localization
extracellular, secreted by the TatAY-TatCY complex PubMed
TatAY-TatCY-dependent export of EfeB requires a functional WprA PubMed
forms a membrane-bound complex with EfeU and EfeO for iron uptake PubMed
attached to the membrane via EfeU PubMed
Expression and Regulation
Operons
Genes
efeU-efeO-efeB
Description
PubMed
Regulation
immediately induced upon iron starvation (first wave to allow iron uptake) (Fur) PubMed
Regulatory mechanism
Fur: repression, PubMed, in fur regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

efeU→efeB

2025-03-08 21:44:13

Jstuelk

105

2df6f8b75b6dc01eab7622f9ec49df472f6aad6f

0A342568538EE157691477116B943A8E96AC21D0

Genes
efeB-ywbO
Description
PubMed
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
SigW: sigma factor, PubMed, in sigW regulon
SigX: sigma factor, in sigX regulon
Open in new tab

efeB→ywbO

2025-03-15 17:22:52

Jstuelk

103

757EB214E95C0222AA8AF39EF6C2953A5B252F94

7259DF3AD73DEA1EF3D0D40B16B47FC14362C226

Biological materials
Mutant
MGNA-B228 (ywbN::erm), available at the NBRP B. subtilis, Japan
BKE38260 (efeB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGTTACAAAACTCCT,  downstream forward: _UP4_TAAAAGAAGCCTTTACAGGC
BKK38260 (efeB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGTTACAAAACTCCT,  downstream forward: _UP4_TAAAAGAAGCCTTTACAGGC
Labs working on this gene/protein
Jan Maarten van Dijl, Groningen, Netherlands
References
Reviews
Loading
Original Publications
Loading

3985F88334D7B034A66473C5BB39188AB61A65F6

Page visits: 6730

Time of last update: 2025-04-06 01:28:51

Author of last update: Jstuelk