Difference between revisions of "CdaR"

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(Original publications)
(Extended information on the protein)
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
** [[CdaA]]-[[CdaR]], to stimulate [[CdaA]] activity {{PubMed|23192352}}  
+
** [[CdaA]]-[[CdaR]], to stimulate [[CdaA]] activity {{PubMed|26240071,23192352}}  
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
 +
** cell membrane, oriented towards the outside {{PubMed|26240071}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 13:05, 5 August 2015

  • Description: effector protein controlling CdaA diadenylate cyclase activity

Gene name cdaR
Synonyms ybbR
Essential no
Product effector protein controlling CdaA diadenylate cyclase activity
Function regulation of c-di-AMP synthesis
Gene expression levels in SubtiExpress: cdaR
Interactions involving this protein in SubtInteract: CdaR
Metabolic function and regulation of this protein in SubtiPathways:
cdaR
MW, pI 52 kDa, 4.719
Gene length, protein length 1449 bp, 483 aa
Immediate neighbours cdaA, glmM
Sequences Protein DNA DNA_with_flanks
Genetic context
YbbR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YbbR expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU01760

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • stimulates CdaA activity upon protein-protein interaction PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: the cdaA-cdaR part of the mRNA is very stable (half-life > 15 min) PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 60 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 138 PubMed

Biological materials

  • Expression vector:
    • IPTG inducible expression of Strep-cdaR in E. coli: pGP2565 (in pGP172), available in Jörg Stülke's lab
    • IPTG inducible expression of His-cdaR in E. coli: pGP2557 (in pET19b), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmids: pGP1989 and pGP1992.

Labs working on this gene/protein

Your additional remarks

References

Reviews

Fabian M Commichau, Achim Dickmanns, Jan Gundlach, Ralf Ficner, Jörg Stülke
A jack of all trades: the multiple roles of the unique essential second messenger cyclic di-AMP.
Mol Microbiol: 2015, 97(2);189-204
[PubMed:25869574] [WorldCat.org] [DOI] (I p)

Original publications