ilvC

ilvC
168

ketol-acid reductoisomerase (2-acetolactate, ketopantoate)

Locus
BSU_28290
Molecular weight
37.30 kDa
Isoelectric point
5.37
Protein length
342 aaSequenceBlast
Gene length
1029 bpSequenceBlast
Function
biosynthesis of branched-chain amino acids and coenzyme A
Product
ketol-acid reductoisomerase (2-acetolactate, ketopantoate)
Essential
no
E.C.
1.1.1.86
Synonyms
ilvC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0059 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,893,681 2,894,709
The protein
Catalyzed reaction/ biological activity
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ --> (2S)-2-acetolactate + H+ + NADPH (according to UniProt)
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ --> (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H+ + NADPH (according to UniProt)
ketopantoate + H+ + NADPH --> (R)-pantoate + NADP+ PubMed
Protein family
ketol-acid reductoisomerase family (single member, according to UniProt)
Domains
N-terminal Rossmann domain (aa 2-181) (according to UniProt)
C-terminal knotted domain (aa 182-327) (according to UniProt)
Structure
4TSK (PDB) (from Alicyclobacillus acidocaldarius, 69.2% identity) PubMed
1NP3 (PDB) (from Pseudomonas aeruginosa, 53% identity, 71% similarity) PubMed
P37253 (AlphaFold)
Modification
phosphorylated on Arg-298 PubMed
phosphorylated on Ser-338 PubMed
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD
Description
PubMed
Regulation
expression is stimulated in the presence of glucose (CcpA) PubMed
Regulatory mechanism
T-box: RNA switch, via tRNA controlled RNA switch, repression by BCAA, in T-box
TnrA: repression, PubMed, in tnrA regulon
CodY: repression, PubMed PubMed, in codY regulon
CcpA: activation, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
An antisense RNA is predicted for leuA PubMed
the T-box RNA is degraded by RNase Y PubMed
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ilvB→leuD

2025-04-04 04:53:41

Jstuelk

148

905a0fc4b9e1945f65cf0820ea8bda45e4ac716c

931C13BE07545B8037A118810CF4C8E29210A7CE

Biological materials
Mutant
BKE28290 (ilvC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCAATCTCTCCCTTGT, downstream forward: _UP4_AAGAAGAAGGAAGCGGTGGT
BKK28290 (ilvC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCAATCTCTCCCTTGT, downstream forward: _UP4_AAGAAGAAGGAAGCGGTGGT
GP3342 (ilvC::lox72), available in Jörg Stülke's lab PubMed
GP4404 (ilvC::neo), available in Jörg Stülke's lab PubMed
LacZ fusion
pGP521 (in pAC5), available in Jörg Stülke's lab
References
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EC1CB2FD563EFDA391D13EB8ADFDC8C9734E9D57

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Time of last update: 2025-04-06 02:58:25

Author of last update: Jstuelk