pucJ

pucJ
168

uric acid permease

Locus
BSU_32430
Molecular weight
46.94 kDa
Isoelectric point
9.64
Protein length
449 aaSequenceBlast
Gene length
1350 bpSequenceBlast
Function
purine utilization
Product
uric acid permease
Essential
no
Synonyms
pucJ, yunJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2233 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,330,502  3,331,851
The protein
Protein family
xanthine/uracil permease family (according to UniProt)
Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily (according to UniProt)
Structure
3QE7 (PDB) (E. coli uracil transporter, 31% identity) PubMed
O32139 (AlphaFold)
Paralogous protein(s)
PbuX, PucK
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
pucJ-pucK-pucL-pucM
Description
PubMed
Regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
Regulatory mechanism
TnrA: activation, PubMed, in tnrA regulon
PucR: activation, PubMed, in pucR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

pucJ→pucM

2025-04-16 02:58:09

ghost

86

c7f8d42ebaa8b9d9f51e20d124bc5b0e15e517cb

00A9C2B4455B613B62244C5080AD61AEB7B11FFE

Genes
pucR-pucJ-pucK-pucL-pucM
Description
PubMed
Regulation
expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
Regulatory mechanism
PucR: auto-repression, PubMed, in pucR regulon
TnrA: activation, PubMed, in tnrA regulon
Open in new tab

pucR→pucM

2025-04-16 03:23:03

ghost

110

276c5386b42b4339a3f84f40bd6b93a74b30984d

76659AC9EBD93756B8415A24C45B3DC60C799944

Biological materials
Mutant
MGNA-A935 (yunJ::erm), available at the NBRP B. subtilis, Japan
BKE32430 (pucJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTATAGTCCCTCCCTGTA,  downstream forward: _UP4_TTAGAAAAAGAGGTGTAAAA
BKK32430 (pucJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTATAGTCCCTCCCTGTA,  downstream forward: _UP4_TTAGAAAAAGAGGTGTAAAA
BP1257(pucJK::cat trp+) available in Jörg Stülke's & Fabian Commichau's lab
References
Loading

53950BFDC128DE46F3F43BDB9D0FDB3D645152DF

Page visits: 5769

Time of last update: 2025-04-16 07:45:23

Author of last update: Melvin.boenninger