mcsB

mcsB
168

catalytic subunit of the McsA-McsB arginine kinase holoenzyme, adaptor protein, forms a gated kinase chamber to mark aberrant bacterial proteins for degradation by ClpC-ClpP, modulator of CtsR-dependent repression

Locus
BSU_00850
Molecular weight
40.97 kDa
Isoelectric point
5.07
Protein length
Gene length
Function
control of CtsR activity
Product
protein arginine kinase
Essential
no
Synonyms
mcsB, yacI

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3869 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
102,484  103,575
Phenotypes of a mutant
more rapid spore germination as compared to wild type cells PubMed
The protein
Catalyzed reaction/ biological activity
phosphorylates and thereby targets non-functional CtsR for degradation by ClpP/ClpC PubMed
phosphorylates and thereby targets MgsR for degradation by ClpP/ClpX PubMed
forms a gated kinase chamber to mark aberrant bacterial proteins for degradation by ClpC-ClpP PubMed
ATP + L-arginyl-[protein] --> ADP + H+ + Nω-phospho-L-arginyl-[protein] (according to UniProt)
Protein family
ATP:guanido phosphotransferase family (single member, according to UniProt)
Phosphagen kinase C-terminal (aa 24-254) (according to UniProt)
Structure
6TV6 (PDB) (octameric McsB) PubMed
6FH1 (PDB) (from Geobacillus stearothermophilus) PubMed
6FH3 (PDB) (pArg-bound state, from Geobacillus stearothermophilus) PubMed
Modification
autophosphorylation on specific arginine residues PubMed, dephosphorylation by YwlE PubMed
autophosphorylation stimulates McsB activity as adaptor protein for ClpC PubMed
cytoplasm (according to UniProt)
Expression and Regulation
Operons
Description
Regulation
expressed during germination and spore outgrowth PubMed
Regulatory mechanism
CtsR: repression, PubMed, in ctsR regulon
Spx: activation, PubMed, in spx regulon
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
SigA: sigma factor, PubMed, in sigA regulon
SigB: sigma factor, PubMed PubMed, in sigB regulon
SigF: sigma factor, PubMed, in sigF regulon
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ctsRdisA

2024-12-18 02:19:19

Bzhu

141

b7066b5ca5004b095502d71420c469e9ee925bfa

163769A1708F45F8ABE642374A97FA8A00293529

Biological materials
Mutant
MGNA-B930 (yacI::erm), available at the NBRP B. subtilis, Japan
mcsB::aphA3 availbale from the Gerth lab
mcsBC167S::spec available from the Gerth lab
GP1457 (mcsB::aphA3),  available in Jörg Stülke's lab
BP69 (spc), available in Fabian Commichau's lab
BKE00850 (mcsB::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CTGAATAAAATGCTTTAGCG,  downstream forward: _UP4_AAAAGACAGGAGGATGAATC
BKK00850 (mcsB::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CTGAATAAAATGCTTTAGCG,  downstream forward: _UP4_AAAAGACAGGAGGATGAATC
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
expression of native mcsB in B. subtilis: pBP186 (in pBQ200), available in Fabian Commichau's lab
Antibody
available in Gerth lab
Labs working on this gene/protein
Ulf Gerth, Greifswald, Germany
References
Reviews
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Original Publications
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7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948

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Time of last update: 2024-12-18 13:23:17

Author of last update: Jstuelk