mcsB
168
catalytic subunit of the McsA-McsB arginine kinase holoenzyme, adaptor protein, forms a gated kinase chamber to mark aberrant bacterial proteins for degradation by ClpC-ClpP, modulator of CtsR-dependent repression
Locus
BSU_00850
Molecular weight
40.97 kDa
Isoelectric point
5.07
Function
control of CtsR activity
Product
protein arginine kinase
Essential
no
Synonyms
mcsB, yacI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3869 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
102,484 103,575
Phenotypes of a mutant
more rapid spore germination as compared to wild type cells PubMed
The protein
Catalyzed reaction/ biological activity
forms a gated kinase chamber to mark aberrant bacterial proteins for degradation by ClpC-ClpP PubMed
ATP + L-arginyl-[protein] --> ADP + H+ + Nω-phospho-L-arginyl-[protein] (according to UniProt)
Protein family
ATP:guanido phosphotransferase family (single member, according to UniProt)
Phosphagen kinase C-terminal (aa 24-254) (according to UniProt)
Structure
6FH1 (PDB) (from Geobacillus stearothermophilus) PubMed
6FH3 (PDB) (pArg-bound state, from Geobacillus stearothermophilus) PubMed
Modification
cytoplasm (according to UniProt)
Expression and Regulation
Operons
Description
Regulation
expressed during germination and spore outgrowth PubMed
Regulatory mechanism
Sigma factors
Open in new tab
Biological materials
Mutant
MGNA-B930 (yacI::erm), available at the NBRP B. subtilis, Japan
mcsB::aphA3 availbale from the Gerth lab
mcsBC167S::spec available from the Gerth lab
GP1457 (mcsB::aphA3), available in Jörg Stülke's lab
BP69 (spc), available in Fabian Commichau's lab
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
expression of native mcsB in B. subtilis: pBP186 (in pBQ200), available in Fabian Commichau's lab
Antibody
available in Gerth lab
Labs working on this gene/protein
Ulf Gerth, Greifswald, Germany
References
Reviews
Loading
Original Publications
Loading
Page visits: 7587
Time of last update: 2024-12-18 13:23:17
Author of last update: Jstuelk