khtT

khtT
168

K+/H+ antiporter for K+ efflux

Locus
BSU_09860
Molecular weight
18.59 kDa
Isoelectric point
4.89
Protein length
165 aaSequenceBlast
Gene length
498 bpSequenceBlast
Function
potassium ion efflux
Product
K+/H+ antiporter for K+ efflux
Essential
no
Synonyms
khtT, yhaT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0490 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,060,427 → 1,060,924
The protein
Domains
contains a RCK_C domain at the C-terminus (according to UniProt, x)
Structure
7AGV (PDB) (complex with c-di-AMP) PubMed
O07535 (AlphaFold)
Effectors of protein activity
binds c-di-AMP, which activates export activity PubMed
Paralogous protein(s)
YrvC
Localization
cell membrane (peripheral) (according to UniProt)
Expression and Regulation
Operons
Genes
khtS-khtT-khtU
Description
PubMed
Open in new tab

khtS→khtU

2025-04-06 05:35:01

ghost

110

54fb18209326e1c8d64853667143b4fd8e7337e1

ACAE2230F2FD54981F6B24985DF9C660B7B5FFBB

Biological materials
Mutant
MGNA-A675 (yhaT::erm), available at the NBRP B. subtilis, Japan
GP2078 (ΔkhtS-khtT-khtU::aphA3), available in Jörg Stülke's lab
BKE09860 (ΔkhtT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT,  downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC
BKK09860 (ΔkhtT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGAAAATCCCTCCGAAT,  downstream forward: _UP4_TCTGGAGAGGGCGTGTGAGC
Expression vectors
pGP2906 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
References
Reviews
Loading
Original Publications
Loading

D8A8FBC05B9665F72A88685D2B33822C2BB84C62

Page visits: 3713

Time of last update: 2025-04-10 21:19:59

Author of last update: Jstuelk