cwlJ

cwlJ
168

spore coat protein, cell wall hydrolase

Locus
BSU_02600
Molecular weight
16.22 kDa
Isoelectric point
9.35
Protein length
142 aaSequenceBlast
Gene length
429 bpSequenceBlast
Function
spore germination
Product
spore coat protein, cell wall hydrolase
Essential
no
E.C.
null
Synonyms
cwlJ, ycbQ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3773 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
282,469  282,897
The protein
Protein family
cwlJ family (single member, according to UniProt)
Structure
P42249 (AlphaFold)
Effectors of protein activity
activity depends on SwsB PubMed
Localization
outer edge of the spore cortex PubMed
inner spore coat, localization depends on SafA, CotE, and GerQ PubMed
Expression and Regulation
Operons
Genes
cwlJ
Description
PubMed
Regulation
expressed during sporulation in the mother cell (SigE, SigK, SpoIIID) PubMed
Regulatory mechanism
SpoIIID: activation, PubMed, in spoIIID regulon
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
SigK: sigma factor, PubMed, in sigK regulon
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cwlJ

2025-03-15 20:23:28

ghost

75

39a528a58c325d5deb55135f4e71e87ba3d91021

3D900C6488D72F096AB39A4F1C57DDF5C358815A

Biological materials
Mutant
1G19 ( cwlJ::tet), PubMed, available at BGSC
BKE02600 (cwlJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACGTCACCTCCCCTTG,  downstream forward: _UP4_TAGAGACAGGACACCGTTCA
BKK02600 (cwlJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACGTCACCTCCCCTTG,  downstream forward: _UP4_TAGAGACAGGACACCGTTCA
References
Reviews
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Original Publications
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49ED84B48CEC09493B6056D03D2A57578770CE15

Page visits: 4685

Time of last update: 2025-04-10 21:18:33

Author of last update: Jstuelk