nagA

nagA
168

N-acetylglucosamine-6-phosphate deacetylase

Locus
BSU_35010
Molecular weight
42.46 kDa
Isoelectric point
5.28
Protein length
396 aaSequenceBlast
Gene length
1191 bpSequenceBlast
Function
N-acetylglucosamine utilization
Product
N-acetylglucosamine-6-phosphate deacetylase
Essential
no
E.C.
3.5.1.25
Synonyms
nagA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1820 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,595,356  3,596,546
Phenotypes of a mutant
no growth on N-acetylglucosamine PubMed
The protein
Catalyzed reaction/ biological activity
H2O + N-acetyl-D-glucosamine 6-phosphate --> acetate + D-glucosamine 6-phosphate (according to UniProt)
Protein family
Metallo-dependent hydrolases superfamily (according to UniProt)
Structure
2VHL (PDB) PubMed
O34450 (AlphaFold)
Kinetic information
K(M): 1.4 mM PubMed
Expression and Regulation
Operons
Genes
nagA-nagB-nagR
Description
PubMed
Regulation
induced in the presence of N-acetylglucosamine (NagR) PubMed
Regulatory mechanism
NagR: repression, PubMed, in nagR regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

nagA→nagR

2025-04-04 08:11:11

ghost

118

dfb9f60c6cb4712882f6e111396691335972d607

2CE10C0E65E696F5F9B1AAC17ED9D19E163A6ECF

Biological materials
Mutant
MGNA-A337 (nagA::erm), available at the NBRP B. subtilis, Japan
BKE35010 (nagA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGATGATCCGCCTTTCT,  downstream forward: _UP4_ATATCCAAGGAGGCTGACCA
BKK35010 (nagA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGATGATCCGCCTTTCT,  downstream forward: _UP4_ATATCCAAGGAGGCTGACCA
References
Reviews
Gilmore MC, Cava FBacterial peptidoglycan recycling.Trends in microbiology. 2024 Nov 28; . PMID: 39613687
Plumbridge J Regulation of the Utilization of Amino Sugars by Escherichia coli and Bacillus subtilis: Same Genes, Different Control. Journal of molecular microbiology and biotechnology. 2015; 25(2-3):154-67. doi:10.1159/000369583. PMID:26159076
Original Publications
Fillenberg SB, Grau FC, Seidel G, Muller YA Structural insight into operator dre-sites recognition and effector binding in the GntR/HutC transcription regulator NagR. Nucleic acids research. 2015 Jan; 43(2):1283-96. doi:10.1093/nar/gku1374. PMID:25564531
Gaugué I, Oberto J, Plumbridge J Regulation of amino sugar utilization in Bacillus subtilis by the GntR family regulators, NagR and GamR. Molecular microbiology. 2014 Apr; 92(1):100-15. doi:10.1111/mmi.12544. PMID:24673833
Liu Y, Liu L, Shin HD, Chen RR, Li J, Du G, Chen J Pathway engineering of Bacillus subtilis for microbial production of N-acetylglucosamine. Metabolic engineering. 2013 Sep; 19:107-15. doi:10.1016/j.ymben.2013.07.002. pii:S1096-7176(13)00066-9. PMID:23876412
Gaugué I, Oberto J, Putzer H, Plumbridge J The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine. PloS one. 2013; 8(5):e63025. doi:10.1371/journal.pone.0063025. PMID:23667565
Bertram R, Rigali S, Wood N, Lulko AT, Kuipers OP, Titgemeyer F Regulon of the N-acetylglucosamine utilization regulator NagR in Bacillus subtilis. Journal of bacteriology. 2011 Jul; 193(14):3525-36. doi:10.1128/JB.00264-11. PMID:21602348
Vincent F, Yates D, Garman E, Davies GJ, Brannigan JA The three-dimensional structure of the N-acetylglucosamine-6-phosphate deacetylase, NagA, from Bacillus subtilis: a member of the urease superfamily. The Journal of biological chemistry. 2004 Jan 23; 279(4):2809-16. . PMID:14557261
BATES CJ, PASTERNAK CA FURTHER STUDIES ON THE REGULATION OF AMINO SUGAR METABOLISM IN BACILLUS SUBTILIS. The Biochemical journal. 1965 Jul; 96:147-54. . PMID:14343123
Blencke HM, Homuth G, Ludwig H, Mäder U, Hecker M, Stülke J Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metabolic engineering. 2003 Apr; 5(2):133-49. . PMID:12850135
Reizer J, Bachem S, Reizer A, Arnaud M, Saier MH, Stülke J Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology (Reading, England). 1999 Dec; 145 ( Pt 12):3419-29. . PMID:10627040
Mobley HL, Doyle RJ, Streips UN, Langemeier SO Transport and incorporation of N-acetyl-D-glucosamine in Bacillus subtilis. Journal of bacteriology. 1982 Apr; 150(1):8-15. . PMID:6174502

8F4D075C9B9EF995E361F9BA5E8B52F42059C422

Page visits: 3570

Time of last update: 2025-04-06 02:39:17

Author of last update: Jstuelk