nagA

nagA
168

N-acetylglucosamine-6-phosphate deacetylase

Locus
BSU_35010
Molecular weight
42.46 kDa
Isoelectric point
5.28
Protein length
396 aaSequenceBlast
Gene length
1191 bpSequenceBlast
Function
N-acetylglucosamine utilization
Product
N-acetylglucosamine-6-phosphate deacetylase
Essential
no
E.C.
3.5.1.25
Synonyms
nagA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1820 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,595,356  3,596,546
Phenotypes of a mutant
no growth on N-acetylglucosamine PubMed
The protein
Catalyzed reaction/ biological activity
H2O + N-acetyl-D-glucosamine 6-phosphate --> acetate + D-glucosamine 6-phosphate (according to UniProt)
Protein family
Metallo-dependent hydrolases superfamily (according to UniProt)
Structure
2VHL (PDB) PubMed
O34450 (AlphaFold)
Kinetic information
K(M): 1.4 mM PubMed
Expression and Regulation
Operons
Genes
nagA-nagB-nagR
Description
PubMed
Regulation
induced in the presence of N-acetylglucosamine (NagR) PubMed
Regulatory mechanism
NagR: repression, PubMed, in nagR regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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nagA→nagR

2025-04-04 08:11:11

ghost

118

dfb9f60c6cb4712882f6e111396691335972d607

2CE10C0E65E696F5F9B1AAC17ED9D19E163A6ECF

Biological materials
Mutant
MGNA-A337 (nagA::erm), available at the NBRP B. subtilis, Japan
BKE35010 (nagA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGATGATCCGCCTTTCT,  downstream forward: _UP4_ATATCCAAGGAGGCTGACCA
BKK35010 (nagA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGATGATCCGCCTTTCT,  downstream forward: _UP4_ATATCCAAGGAGGCTGACCA
References
Reviews
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Original Publications
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8F4D075C9B9EF995E361F9BA5E8B52F42059C422

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Time of last update: 2025-04-05 01:04:25

Author of last update: Jstuelk