hag

hag
168

flagellin protein, about 12,000 subunits make up one flagellum

Locus
BSU_35360
Molecular weight
32.47 kDa
Isoelectric point
4.78
Protein length
304 aaSequenceBlast
Gene length
915 bpSequenceBlast
Function
motility and chemotaxis
Product
flagellin protein
Essential
no
Synonyms
hag
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1344 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,634,987 3,635,901
Phenotypes of a mutant
increased DegU-P formation, resulting in reduced comK expression and strongly reduced genetic competence PubMed
defective in Swarming motility PubMed
not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
few hours delay in pellicle development PubMed
mutant has increased fitness in planktonic culture when competed with the wild-type NCIB3610 strain PubMed
a substitution of Ala233 to Val results in straight flagella PubMed
Additional information
the hag gene is often inactivated during adaptation to plants PubMed
The protein
Protein family
bacterial flagellin family (with FlgL and YvzB, according to UniProt)
Structure
3A5X (PDB) (from Salmonella typhimurium, 42% identity) PubMed
the structure of flagellar filaments: PubMed
P02968 (AlphaFold)
Paralogous protein(s)
YvzB, (C-terminal domain)
Localization
extracellular (no signal peptide), major component of the secretome PubMed
membrane PubMed
Additional information
belongs to the 100 most abundant proteins PubMed
12.000 monomers make up one flagellum PubMed
Expression and Regulation
Operons
Genes
hag
Description
PubMed
Regulation
repressed during growth in the presence of branched chain amino acids (CodY) PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
CodY: repression, PubMed, in codY regulon
ScoC: repression, PubMed, in scoC regulon
Sigma factors
SigD: sigma factor, PubMed, in sigD regulon
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hag

2025-03-16 09:25:09

Jstuelk

122

AAE70DC342AC480A6373EFD3C44ECF03EC296898

BC109737D2CD641FC2627FCABBE6884EEC045B18

Other regulations
CsrA: translation inhibition, PubMed
Biological materials
Mutant
GP901 (aphA3), GP902 (tet) PubMed, both available in Jrg Stlke's lab
1A915 ( hag::cat), PubMed, available at BGSC
1A783 ( hag::erm), PubMed, available at BGSC
1A842 ( hag::kan), PubMed, available at BGSC
DS1677 (marker-less in NCIB3610) PubMed
BKE35360 (hag::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTTTTGTTCCTCCCTG, downstream forward: _UP4_TAATTTTAAAAAAGACCTTG
BKK35360 (hag::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTTTTGTTCCTCCCTG, downstream forward: _UP4_TAATTTTAAAAAAGACCTTG
Expression vectors
expression in B. subtilis, in pBQ200: pGP1089, available in Jrg Stlke's lab
LacZ fusion
pGP1035 (in pAC6), pGP755 (in pAC7), there is also a series of promoter deletion variants in pAC6 and pAC7 PubMed, all available in Jrg Stlke's lab
GFP fusion
BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in Jrg Stlke's lab
Labs working on this gene/protein
Daniel Kearns
References
Reviews
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Original Publications
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E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB

Page visits: 9850

Time of last update: 2025-04-03 16:17:11

Author of last update: Jstuelk