cheD

cheD
168

chemoreceptor deaminase, required for methylation of methyl-accepting chemotaxis proteins by CheR, enhances phosphatase activity of CheC, required for full McpC activity

Locus
BSU_16460
Molecular weight
17.86 kDa
Isoelectric point
9.35
Protein length
166 aaSequenceBlast
Gene length
501 bpSequenceBlast
Function
motility and chemotaxis
Product
protein deaminase, CheC activity modulator
Essential
no
E.C.
3.5.1.44
Synonyms
cheD, ylxK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1871 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,715,970 → 1,716,470
Phenotypes of a mutant
not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
defective in Swarming motility PubMed
The protein
Catalyzed reaction/ biological activity
binds chemoreceptors and increases their ability to activate the kinase activity of CheA PubMed
H2O + L-glutaminyl-[protein] --> L-glutamyl-[protein] + NH4+ (according to UniProt)
deaminates Gln-586, Gln-593, and Gln594 in McpA PubMed
deaminates Gln-609 in McpC PubMed
Protein family
CheD family (single member, according to UniProt)
Structure
2F9Z (PDB) (from Thermotoga maritima, 41% identity) PubMed
P40404 (AlphaFold)
Expression and Regulation
Operons
Genes
ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
see fla-che operon
Regulatory mechanism
Spo0A: repression, in spo0A regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SwrA: activation, in swrA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
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ylxF→swrB

2025-04-04 21:21:07

Jstuelk

170

EECFE9022742ECC57D9BBA5CE7DB7A4D1BFD42A8

0535E3D13CBD000A94D62050515BF8D1D5E3F011

Genes
flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
Description
PubMed
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
Regulatory mechanism
Spo0A: repression, in spo0A regulon
SwrA: activation, in swrA regulon
CodY: repression, in codY regulon
DegU: repression, in degU regulon
SinR: transcription elongation, binding of the SinR-SlrR heteromer to sites within fliE and fliI results in inhibition of transcription elongation PubMed, in sinR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
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flgB→swrB

2025-04-03 01:56:48

Jstuelk

173

024d59ae468aa14568753f57122b789c39ed6c06

4D920B925DA071AE7390E022B9AB625E40B7B2AC

Biological materials
Mutant
1A861 ( cheD::cat), PubMed, available at BGSC
1A863 ( cheD::cat), PubMed, available at BGSC
DS6868 (marker-less in NCIB3610) PubMed
BKE16460 (ΔcheD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TATAACAACAGCCTCAGTTG,  downstream forward: _UP4_TAATTAAGGTATTAGGGGGA
BKK16460 (ΔcheD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TATAACAACAGCCTCAGTTG,  downstream forward: _UP4_TAATTAAGGTATTAGGGGGA
References
Reviews
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Original Publications
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BE9373BEB682E5F8B0938AAA4ED9B723B357ABAE

Page visits: 4139

Time of last update: 2025-04-05 02:31:32

Author of last update: Jstuelk