pcrA

pcrA
168

ATP-dependent DNA helicase, coupling of DNA replication, transcription, recombination and segregation, facilitates unwinding of ICEBs1 DNA for horizontal transfer, required to overcome a replicative stress

Locus
BSU_06610
Molecular weight
83.37 kDa
Isoelectric point
5.77
Protein length
739 aaSequenceBlast
Gene length
2220 bpSequenceBlast
Function
coupling of DNA replication, transcription, recombination and segregation
Product
ATP-dependent DNA helicase
Essential
yes
Synonyms
pcrA, yerF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0210 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
719,370 → 721,589
Phenotypes of a mutant
essential PubMed
inactivation can be suppressed by inactivation of either recA, recJ, recO, or mfd PubMed
inactivation can be partially suppressed by inactivation of rnhB PubMed
The protein
Catalyzed reaction/ biological activity
coupling of DNA replication, transcription, recombination and segregation PubMed
PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop PubMed
dismantles RecA filaments PubMed
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
preferentially unwinds ssDNA in the 3′→ 5′ direction PubMed
unwinds DNA-RNA hybrids PubMed
Protein family
helicase family (according to UniProt)
Domains
two RecA-like domains PubMed
intrinxically disordered C-terminal domain (for interaction with RNA polymerase) PubMed
UvrD-like helicase ATP-binding domain(aa 10-289) (according to UniProt)
UvrD-like helicase C-terminal (aa 290-570) (according to UniProt)
Structure
2PJR (PDB) (product complex, complex with sulphate ion, Geobacillus stearothermophilus)
1PJR (PDB) (substrate complex, complex with DNA, Geobacillus stearothermophilus)
5DMA (PDB), C-terminal RNA polymerase interaction domain PubMed
O34580 (AlphaFold)
Effectors of protein activity
unwinding of the double-stranded ICEBs1 DNA is stimulated by HelP PubMed
helicase activity is stimulated by interaction with RNA polymerase PubMed
Paralogous protein(s)
YjcD
Expression and Regulation
Operons
Genes
pcrB-pcrA-ligA-yerH
Description
PubMed
Regulation
induced by DNA damage (LexA) PubMed
Regulatory mechanism
LexA: repression, PubMed, in lexA regulon
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pcrB→yerH

2025-04-10 00:10:33

ghost

142

dec907ab37dccb52bc69c4b0f73a394d7c7ee297

FEC0EF466BE2820083D3AF39105152A266EEF005

Biological materials
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
References
Reviews
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Original Publications
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C73E52D214E24F21696973B19B0A44CE785D6FBA

Page visits: 5445

Time of last update: 2025-04-10 09:09:01

Author of last update: Jstuelk