sda

sda
168

developmental checkpoint protein, controls the phosphorylation status of Spo0A

Locus
BSU_25690
Molecular weight
6.02 kDa
Isoelectric point
5.61
Protein length
52 aaSequenceBlast
Gene length
159 bpSequenceBlast
Function
mediates a developmental checkpoint coupling initiation of Sporulation (phosphorylation of Spo0A)to the function of replication initiation proteins
Product
developmental checkpoint protein
Essential
no
Synonyms
sda
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5851 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,647,456 → 2,647,614
Phenotypes of a mutant
sporulates at a higher frequency
will sporulate in the presence of replication stress
The protein
Catalyzed reaction/ biological activity
Sda is a checkpoint protein that is upregulated in response to replication stress PubMed
Sda inhibits the autokinase activity of KinA (and likely KinB as well). This in its turn results in reduced levels of Spo0A~P]]. Thus, replication stressed cells will not initiate sporulation.
Structure
3D36 (PDB) (from Geobacillus stearothermophilus, complex with KinB) PubMed,  1PV0 (PDB)
Q7WY62 (AlphaFold)
Expression and Regulation
Operons
Genes
sda
Description
PubMed
Regulation
induced by DNA damage (LexA) PubMed
Regulatory mechanism
DnaA: activation, under replication stress PubMed, in dnaA regulon
LexA: repression, PubMed, in lexA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
An antisense RNA is predicted for 'sda' PubMed
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sda

2025-03-16 04:00:59

ghost

69

afe15634e2c434b063a5ca259e194111a63c6588

A8DD776891B451FB5BF1EFE094629AB228D8C1B1

Additional information
translation of Sda is reduced during serine starvation due to serine codon hierarchy-mediated regulation PubMed
Biological materials
Mutant
1A1034 (no resistance), PubMed, available at BGSC
BKE25690 (Δsda::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATGGGAGAGGCACCTCCTT,  downstream forward: _UP4_TAATAGGAATTTGTCTATTT
BKK25690 (Δsda::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATGGGAGAGGCACCTCCTT,  downstream forward: _UP4_TAATAGGAATTTGTCTATTT
Labs working on this gene/protein
Bill Burkholder Homepage
References
Reviews
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Original Publications
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CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611

Page visits: 4004

Time of last update: 2025-04-04 01:49:53

Author of last update: Jstuelk