Sda

• Description: developmental checkpoint protein, inhibits the autokinase activity of KinA and KinB, control of the phosphorelay

Gene name	sda
Synonyms
Essential	no
Product	developmental checkpoint protein
Function	mediates a developmental checkpoint coupling initiation of sporulation (phosphorylation of Spo0A) to the function of replication initiation proteins
Gene expression levels in SubtiExpress: sda
Interactions involving this protein in SubtInteract: Sda
Function and regulation of this protein in SubtiPathways: sda
MW, pI	6 kDa, 5.605
Gene length, protein length	156 bp, 52 aa
Immediate neighbours	yloV, yqeF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphorelay

This gene is a member of the following regulons

DnaA regulon, LexA regulon

The gene

Basic information

• Locus tag: BSU25690

Phenotypes of a mutant

• sporulates at a higher frequency
• will sporulate in the presence of replication stress

Database entries

• BsubCyc: BSU25690

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Sda is a checkpoint protein that is upregulated in response to replication stress PubMed. Sda inhibits the autokinase activity of KinA (and likely KinB as well). This in its turn results in reduced levels of Spo0A~P. Thus, replication stressed cells will not initiate sporulation.

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • KinB-Sda PubMed
  • KinA-Sda PubMed

• Localization:

Database entries

• BsubCyc: BSU25690

• Structure: 3D36 (from Geobacillus stearothermophilus, complex with KinB) PubMed, 1PV0

• UniProt: Q7WY62

• KEGG entry: [3]

• E.C. number:

Additional information

• Sda is rapidly degraded by the ClpP/X system because of some uncharged residues at its C-terminus PubMed

Expression and regulation

• Operon: sda PubMed

• Expression browser: sda PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • DnaA: transcription activation under replication stress PubMed
  • LexA: transcription repression PubMed

• Additional information:
  • Sda is rapidly turned over by the ClpP/X system because of some uncharged residues at its C-terminus PubMed.
  • An antisense RNA is predicted for sda PubMed

Biological materials

• Mutant:
  • 1A1034 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Bill Burkholder Homepage

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)