cwlT
168

cell wall hydrolase, required for conjugation of ICEBs1, part of the type IV secretion system for DNA transfer , C-terminal domain hydrolyzes bond between D-Glu and m-DAP

Locus

BSU_04970

Molecular weight

36.39 kDa

Isoelectric point

8.63

Protein length

329 aaSequenceBlast

Gene length

990 bpSequenceBlast

Function

conjugative transfer of ICEBs1

Product

cell wall hydrolase

Synonyms

cwlT, yddH

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0791 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

544,022 545,011

The protein

Catalyzed reaction/ biological activity

hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D--glutamate and meso-diaminopimelic acid) PubMed

degradation of gamma-polyglutamic acid PubMed

Protein family

Peptidase C40 family (according to UniProt)

Domains

N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine PubMed

C-terminal D,L-endopeptidase domain (NlpC/P60 domain) PubMed, cleaves the bond between D--glutamate and meso-diaminopimelic acid PubMed

Structure

4FDY (PDB) (from Staphylococcus aureus, 42% identity) PubMed

P96645 (AlphaFold)

Effectors of protein activity

both enzymatic activities are inhibited by interaction with IseA PubMed

Localization