bshC

bshC
168

cysteine-adding enzyme required for the synthesis of bacillithiol

Locus
BSU_15120
Molecular weight
62.44 kDa
Isoelectric point
5.25
Protein length
539 aaSequenceBlast
Gene length
1620 bpSequenceBlast
Function
biosynthesis of bacillithiol
Product
cysteine-adding enzyme
Essential
no
Synonyms
bshC, yllA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4365 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,578,376  1,579,995
Phenotypes of a mutant
impaired growth in minimal medium, reduced activity of enzymes containing FeS cluster PubMed
sensitivity to superoxide (paraquate) stress PubMed
The protein
Protein family
BshC family (single member, according to UniProt)
Structure
4WBD (PDB) PubMed
P55342 (AlphaFold)
Expression and Regulation
Operons
Genes
panE-bshC
Description
PubMed
Regulation
''ylbQ'': induced by diamide stess (thiol depletion) (Spx) PubMed
Regulatory mechanism
Spx: activation, PubMed, in spx regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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panE→bshC

2025-04-12 01:40:36

ghost

102

5167d69519c79cbaec57ca3a2697ba19d8c2a92d

EAD0EECB1A87533845507CEC4FCC017629071F4A

Biological materials
Mutant
MGNA-B128 (bshC::erm), available at the NBRP B. subtilis, Japan
a bshC null mutant is available in John Helmanns lab
BKE15120 (bshC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTCTAGAACTTCCTTT,  downstream forward: _UP4_TGAAATAAAGTTTTAAAGAA
BKK15120 (bshC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTCTAGAACTTCCTTT,  downstream forward: _UP4_TGAAATAAAGTTTTAAAGAA
BP953 (bshC::cat (pdxS-pdxT)::tet), available in Fabian Commichau's lab,  PubMed
References
Reviews
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Original Publications
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115FA333FAD17ACE5EBF288F16F33C330C6B4473

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Time of last update: 2025-04-14 08:02:29

Author of last update: Melvin.boenninger