tasA

tasA
168

major component of biofilm matrix, forms bundles of fibres

Locus
BSU_24620
Molecular weight
28.15 kDa
Isoelectric point
5.44
Protein length
261 aaSequenceBlast
Gene length
786 bpSequenceBlast
Function
biofilm formation
Product
major component of biofilm matrix
Essential
no
Synonyms
tasA, cotN, yqhF
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5884 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,553,081 → 2,553,866
Phenotypes of a mutant
altered cell death pattern in colonies PubMed
increases membrane fluidity and cell death PubMed
failure to produce structured and wrinkled colonies PubMed
The protein
Catalyzed reaction/ biological activity
forms amyloid fibers that bind cells together in the biofilm PubMed
Protein family
peptidase M73 family (single member, according to UniProt)
Structure
5OF1 (PDB) PubMed
5OF2 (PDB) PubMed
P54507 (AlphaFold)
Localization
extracellular (signal peptide) PubMed, secretion requires SipW PubMed
Expression and Regulation
Operons
Genes
tapA-sipW-tasA
Description
PubMed
Regulation
repressed by casamino acids PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
SinR: repression, PubMed, in sinR regulon
RemA: activation, PubMed, in remA regulon
LutR: activation, PubMed, in lutR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
induction by sequestration of SinR by SinI or SlrA PubMed or by SlrR PubMed
expression of the operon is localized to a ring near the periphery of the biofilm PubMed
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tapA→tasA

2025-04-05 17:22:14

Jstuelk

153

5d4b2839ae781570552ba6a28b48e7489d628dd2

25F0FAE210D5101B1935E4625742633C173FC509

Additional information
the mRNA is cleaved by RNase III PubMed
Biological materials
Mutant
MGNA-C449 (yqhF::erm), available at the NBRP B. subtilis, Japan
BKE24620 (ΔtasA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTAAGCTCCCCTTTTA,  downstream forward: _UP4_TAATAACAGCAAAAAAAAGA
BKK24620 (ΔtasA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTAAGCTCCCCTTTTA,  downstream forward: _UP4_TAATAACAGCAAAAAAAAGA
References
Reviews
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Original Publications
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BF97457E986656E4A9FE7A858F5BDF1759850D5C

Page visits: 14885

Time of last update: 2025-04-06 03:45:14

Author of last update: Jstuelk