rplV

rplV
168

ribosomal protein uL22, important for folding and stabilization of the conformation of 23S rRNA

Locus
BSU_01210
Molecular weight
12.32 kDa
Isoelectric point
11.23
Protein length
113 aaSequenceBlast
Gene length
342 bpSequenceBlast
Function
translation
Product
ribosomal protein L22 (uL22)
Essential
no
Synonyms
rplV
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0091 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
138,497 → 138,838
Phenotypes of a mutant
essential PubMed
loop duplication (94SQINKRT100) results in resistance against chloramphenicol, but also to impaired growth under conditions of Mg(2+) limitation PubMed
loop duplication results in increased growth rate PubMed
loop duplication results in reduced hyperpolarization of cells PubMed
loop duplication results in increased expression of mgtE PubMed
The protein
Protein family
universal ribosomal protein uL22 family (single member, according to UniProt)
Structure
3J9W (PDB) (the ribosome) PubMed
P42060 (AlphaFold)
Localization
ribosome
Expression and Regulation
Operons
Genes
rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-oapB-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ
Description
PubMed
Regulation
strongly repressed in response to glucose starvation in M9 medium PubMed
likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
Sigma factors
SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon
Additional information
term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed
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rpsJ→rplQ

2025-04-02 13:05:40

Jstuelk

147

911D53E804EEFE6335D6A0F79722F9870F387CD3

9027854E648E8BF3E4A3C8492D350C69AC71C218

Biological materials
Mutant
BKE01210 (ΔrplV::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTAAAAGCCTCCTCTCT,  downstream forward: _UP4_TCAGAAAAGAAGGAGGGATA
References
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F3DE42C41EF57DC5DFFF0D28129F2CE5DB781D99

Page visits: 5484

Time of last update: 2025-04-06 07:00:45

Author of last update: Jstuelk