yerD

yerD
168

general stress protein, similar to glutamate synthase (ferredoxin), required for protection against paraquat stress

Locus
BSU_06590
Molecular weight
58.58 kDa
Isoelectric point
7.35
Protein length
525 aaSequenceBlast
Gene length
1578 bpSequenceBlast
Function
protection against paraquat stress
Product
unknown
Essential
no
Synonyms
yerD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0069 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
716,780 → 718,357
The protein
Protein family
glutamate synthase family (with GltA and GltB, according to UniProt)
Cofactors
FMN or FAD PubMed
Structure
1EA0 (PDB) (from Azospirillum brasilense, corresponds to aa 184 ... 495, 30% identity)
O34849 (AlphaFold)
Paralogous protein(s)
GltA
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
yerD
Description
PubMed
Regulation
induced by stress (SigB) PubMed
Sigma factors
SigB: sigma factor, PubMed, in sigB regulon
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yerD

2025-04-05 23:45:11

ghost

83

8a7c5306a68e91da05898da41672c2a7ea73f79f

21A32946C8BFCE13E4005C8696FD6CF2A4CA674A

Biological materials
Mutant
MGNA-A925 (yerD::erm), available at the NBRP B. subtilis, Japan
GP1184 (tet) available in Jörg Stülke's lab
BKE06590 (ΔyerD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTCAGCCTCCCTTTT,  downstream forward: _UP4_TAAAGAAACCGCCCTTGCCA
BKK06590 (ΔyerD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTCAGCCTCCCTTTT,  downstream forward: _UP4_TAAAGAAACCGCCCTTGCCA
Expression vectors
pBP32 (expression vector for E. coli BL21; N-terminal Strep-tag-YerD fusion protein, based on pGP172), available in Fabian Commichau's lab
FLAG-tag construct
GP1187 (spc, based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Fabian Commichau, Göttingen, Germany homepage
References
de Jong IG, Veening JW, Kuipers OP Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation. Environmental microbiology. 2012 Dec; 14(12):3110-21. doi:10.1111/j.1462-2920.2012.02892.x. PMID:23033921
Reder A, Höper D, Gerth U, Hecker M Contributions of individual σB-dependent general stress genes to oxidative stress resistance of Bacillus subtilis. Journal of bacteriology. 2012 Jul; 194(14):3601-10. doi:10.1128/JB.00528-12. PMID:22582280
Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis. Science (New York, N.Y.). 2012 Mar 02; 335(6072):1103-6. doi:10.1126/science.1206848. PMID:22383849
Höper D, Völker U, Hecker M Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis. Journal of bacteriology. 2005 Apr; 187(8):2810-26. . PMID:15805528

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Time of last update: 2025-04-10 12:06:03

Author of last update: Jstuelk