metE

metE
168

methionine synthase

Locus
BSU_13180
Molecular weight
86.64 kDa
Isoelectric point
4.84
Protein length
762 aaSequenceBlast
Gene length
2289 bpSequenceBlast
Function
biosynthesis of methionine
Product
methionine synthase
Essential
no
E.C.
2.1.1.14
Synonyms
metE, metC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0620 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,383,320 1,385,608
The protein
Catalyzed reaction/ biological activity
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine --> L-methionine + tetrahydropteroyltri-L-glutamate (according to UniProt)
Protein family
vitamin-B12 independent methionine synthase family (single member, according to UniProt)
Structure
1T7L (PDB) (from Thermotoga maritima, 44% identity, 61% similarity) PubMed
P80877 (AlphaFold)
Modification
phosphorylated on ser/ thr/ tyr PubMed, S-cysteinylation after diamide stress (C719) PubMed
Cys719 and Cys730 are S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed
MetE is generally most strongly S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed
Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMed
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
metE
Regulation
repressed by casamino acids PubMed
the S-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box
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metE

2025-04-01 19:35:31

Jstuelk

96

a2c1a26ddf575e47d02ea8b1f4d126209fdafc94

CDE3048378AB7CEEA67FEC21DE6642EF6492A3D2

Biological materials
Mutant
1A607 ( metE::erm), PubMed, available at BGSC
BKE13180 (metE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTTTCTCCTCCTTTAT, downstream forward: _UP4_TAATTTGAAAAAACCATCTG
BKK13180 (metE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTTTCTCCTCCTTTAT, downstream forward: _UP4_TAATTTGAAAAAACCATCTG
References
Reviews
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Original Publications
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23F405D5B849E4EDC1D599F86C71DFD2E55C0305

Page visits: 3986

Time of last update: 2025-04-06 06:00:16

Author of last update: Melvin.boenninger