ksgA
168

rRNA adenine dimethyltransferase

Locus

BSU_00420

Molecular weight

32.57 kDa

Isoelectric point

5.71

Protein length

292 aaSequenceBlast

Gene length

879 bpSequenceBlast

Function

rRNA maturation

Product

rRNA adenine dimethyltransferase

Essential

E.C.

2.1.1.-

Synonyms

ksgA

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0030 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

50,640 → 51,518

Phenotypes of a mutant

defective in swarming motility (reduced rate of colony expansion) PubMed

The protein

Catalyzed reaction/ biological activity

adenosine¹⁵¹⁸/adenosine¹⁵¹⁹ in 16S rRNA + 4 S-adenosyl-L-methionine --> 4 H⁺ + N⁶-dimethyladenosine¹⁵¹⁸/N⁶-dimethyladenosine¹⁵¹⁹ in 16S rRNA + 4 S-adenosyl-L-homocysteine (according to UniProt)

Protein family

Methyltransferase superfamily (according to UniProt)

class I-like SAM-binding methyltransferase superfamily (according to UniProt)

Structure

6IFT (PDB) (complexed with S-adenosylmethionine) PubMed

6IFS (PDB) PubMed

6IFV (PDB) (C-terminal truncation) PubMed

6IFW (PDB) (Chimeric protein) PubMed

P37468 (AlphaFold)

Localization

cytoplasm (according to UniProt)

Expression and Regulation

Operons

Genes

rnmV-ksgA

Description

PubMed

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Biological materials

Mutant

MGNA-B907 (ksgA::erm), available at the NBRP B. subtilis, Japan

BKE00420 (ΔksgA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTTTATTCATTCTGTTCCTC, downstream forward: _UP4_TAAAAGGGCTTTTTGTTTTG

BKK00420 (ΔksgA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTTTATTCATTCTGTTCCTC, downstream forward: _UP4_TAAAAGGGCTTTTTGTTTTG

References

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Time of last update: 2025-04-10 07:27:39

Author of last update: Jstuelk

ksgA 168

ksgA
168