mutM

mutM
168

formamidopyrimidine-DNA glycosidase, part of the error prevention oxidized guanine system (with MutY and RppH)

Locus
BSU_29080
Molecular weight
31.06 kDa
Isoelectric point
8.89
Protein length
276 aaSequenceBlast
Gene length
831 bpSequenceBlast
Function
DNA repair
Product
formamidopyrimidine-DNA glycosidase
Essential
no
E.C.
3.2.2.23
Synonyms
mutM, ytaE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0266 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,972,329 → 2,973,159
Phenotypes of a mutant
increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage PubMed
absence of MutM promotes mutagenesis allowing nutritionally stressed B. subtilis cells to escape from growth limiting conditions PubMed
increased sponteous mutation rate PubMed
The protein
Catalyzed reaction/ biological activity
binds to abasic and RNA base DNA lesions PubMed
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine (according to UniProt)
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to UniProt)
Protein family
FPG family (single member, according to UniProt)
Structure
2F5S (PDB) (complex with oxoG:C, Geobacillus stearothermophilus)
O34403 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
polA-mutM-ytaF-coaE
Description
PubMed
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
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polA->coaE

2025-04-03 07:01:21

Bzhu

112

a75923d1aa25d6709b0150853eba1995ea3a02b6

22EE8CEC7C4AE4A2CFE080A9E0FF1AE943C8B023

Biological materials
Mutant
MGNA-A116 (mutM::erm), available at the NBRP B. subtilis, Japan
BKE29080 (ΔmutM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATCCATCACTTCCTATC,  downstream forward: _UP4_TAGCATAATGCTTCATTGTC
BKK29080 (ΔmutM::kan  trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_CACATCCATCACTTCCTATC,  downstream forward: _UP4_TAGCATAATGCTTCATTGTC
References
Reviews
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Original Publications
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EC96EDED2472607DFA4C5EBA054157F7832EE41C

Page visits: 3555

Time of last update: 2025-04-06 01:24:35

Author of last update: Jstuelk