Difference between revisions of "Nos"

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=References=
 
=References=
<pubmed>12220171,11856757,20006999 , 21599925 22119809 23943262 21310962,21921039 25194416</pubmed>
+
==Reviews==
 +
<pubmed>9818193</pubmed>
 +
==Original publications==
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<pubmed>12220171,11856757,20006999 , 21599925 22119809 23943262 21310962,21921039 25194416 25826316</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:36, 1 April 2015

  • Description: nitric-oxide synthase

Gene name nos
Synonyms yflM
Essential no
Product nitric-oxide synthase
Function production of nitric oxide
Gene expression levels in SubtiExpress: nos
Interactions involving this protein in SubtInteract: Nos
MW, pI 38 kDa, 5.528
Gene length, protein length 1008 bp, 336 aa
Immediate neighbours yflN, yflL
Sequences Protein DNA DNA_with_flanks
Genetic context
YflM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Nos expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU07630

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • NADPH-dependent oxidation of l-arginine into l-citrulline PubMed
  • Protein family: Bacterial NOS oxygenase subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 2AMO (loose dimer), 1M7V (bound to tetrahydrofolate and arginine)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information: Note that the gene yflL is located between nos and yflK, but on the opposite strand.
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 18 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

M A Marletta, A R Hurshman, K M Rusche
Catalysis by nitric oxide synthase.
Curr Opin Chem Biol: 1998, 2(5);656-63
[PubMed:9818193] [WorldCat.org] [DOI] (P p)

Original publications