proA
168

glutamate-5-semialdehyde dehydrogenase, required for normal and osmoadaptive proline biosynthesis

Locus

BSU_13130

Molecular weight

45.17 kDa

Isoelectric point

5.1

Protein length

415 aaSequenceBlast

Gene length

1248 bpSequenceBlast

Function

biosynthesis of proline

Product

glutamate-5-semialdehyde dehydrogenase

Essential

E.C.

1.2.1.41

Synonyms

proA

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0014 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

1,379,605 1,380,852

Phenotypes of a mutant

auxotrophic for proline PubMed, this can be bypassed by suppressor mutations that allow overexpression of RocD (e.g. constitutive transcription activation by RocR, generation of a SigA-dependent promoter upstream of rocD) PubMed

not viable under conditions of osmotic stress (0.8 M NaCl) PubMed, this can be bypassed by mutations that allow overexpression of the argC-argJ-argB-argD-carA-carB-argF operon resulting from inactivation of ahrC or the AhrC binding site in front of the operon) PubMed

The protein

Catalyzed reaction/ biological activity

L-glutamate 5-semialdehyde + NADP⁺ + phosphate --> H⁺ + L-glutamyl 5-phosphate + NADPH (according to UniProt)

Protein family

gamma-glutamyl phosphate reductase family (single member, according to UniProt)

Structure

1O20 (PDB) (from Thermotoga maritima, 46% identity, 67% similarity) PubMed

P39821 (AlphaFold)

Localization

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons