nahA

nahA
168

RNA pyrophosphohydrolase, converts primary transcripts to 5’-monophosphate RNA

Locus
BSU_34780
Molecular weight
18.07 kDa
Isoelectric point
4.99
Protein length
158 aaSequenceBlast
Gene length
477 bpSequenceBlast
Function
initiation of RNA degradation
Product
RNA pyrophosphohydrolase
Essential
no
Synonyms
nahA, yvcI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1051 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,572,413  3,572,889
The protein
Catalyzed reaction/ biological activity
converts primary transcripts to 5’-monophospate RNA PubMed
prefers G-initiating  RNAs  and required at least one unpaired nucleotide at the 5’-end  of  its  substrates, with  the  5’-terminal nucleotide determining whether primarily ortho- or pyrophosphate is released PubMed
Protein family
Nudix hydrolase (according to UniProt)
Domains
Nudix hydrolase domain (aa 3-130) (according to UniProt)
Cofactors
Mn2+ PubMed
Structure
3FK9 (PDB) (the protein from B. halodurans, 59% identity, 82% similarity)
O06972 (AlphaFold)
Expression and Regulation
Operons
Genes
nahA-yvcJ-glmR-whiA-crh-yvcN
Description
PubMed
Regulation
constitutive expression at both protein and RNA levels PubMed
Expression
in Enterococcus faecalis: induced upon cell wall stress (vancomycin) (WhiA) PubMed
Regulatory mechanism
WhiA: transcription repression, described for Enterococcus faecalis PubMed, in whiA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
A ncRNA is predicted between 'yvcI' and 'TrxB' PubMed
Open in new tab

nahA→yvcN

2025-04-03 12:06:53

Jstuelk

154

BCC5699F6256B8A1B7FD8A979DBBBC9D4D1198AB

9244FB113E998572106D983A19AB3AC81B9F9D35

Biological materials
Mutant
MGNA-B642 (yvcI::erm), available at the NBRP B. subtilis, Japan
BKE34780 (ΔnahA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCTAACCTTCGTCCTGTC,  downstream forward: _UP4_TAGAAAGACAAGTCAGGGGG
BKK34780 (ΔnahA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCTAACCTTCGTCCTGTC,  downstream forward: _UP4_TAGAAAGACAAGTCAGGGGG
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
References
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0C40455EB53D25363FC3EB0E84502A76520F5F91

Page visits: 6476

Time of last update: 2025-04-03 20:58:32

Author of last update: Christoph.elfmann