glmR

glmR
168

accessory uridyltransferase, regulator of carbon partitioning between central metabolism and peptidoglycan biosynthesis, control of cell shape, required for correct localization of PBP1

Locus
BSU_34760
Molecular weight
34.53 kDa
Isoelectric point
5.41
Protein length
317 aaSequenceBlast
Gene length
954 bpSequenceBlast
Function
regulation of carbon partitioning between central metabolism and peptidoglycan biosynthesis, localization of PBP1
Product
activator of GlmS activity
Essential
no
Synonyms
glmR, yvcK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0391 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,570,546  3,571,499
Phenotypes of a mutant
unable to grow with gluconeogenic substrates as single carbon source, this can be suppressed by mutations in cggR, zwf, pgpH, ltaS, yfnI, upon overexpression of glmS or by a point mutation in PgcA (G47S) that results in increased phosphoglucosamine mutase activity PubMed
filamentous or L-shape-like aberrant morphologies (suppressed by Mg2+) PubMed, this is supressed by overexpression of MreB or by deletion of PBP1, ltaS, or yfnI PubMed
a pgcA glmR double mutant is not viable, this can be suppressed by overexpression of GlmM PubMed
inactivation suppresses the essentiality of mbl PubMed
The protein
Catalyzed reaction/ biological activity
GlcNAc-1P + UTP --> UDP-N-acetylglucosamine PubMed
stimulates GlmS activity to facilitate the diversion of carbon from fructose-6-phosphate to peptidoglycan synthesis PubMed
interacts with YvcJ to facilitate genetic transformation PubMed
Protein family
gluconeogenesis factor family (single member, according to UniProt)
Domains
phosphorylated on Thr-304 by PrkC, dephosphorylated by PrpC PubMed
Structure
2O2Z (PDB) (the protein from B. halodurans, 63% identity, 86% similarity)
O06974 (AlphaFold)
Effectors of protein activity
binds UDP-GlcNAc PubMed, this prevents the interaction with GlmS and stimulates interaction with YvcJ PubMed
Localization
localized as a helical-like pattern PubMed
Expression and Regulation
Operons
Genes
nahA-yvcJ-glmR-whiA-crh-yvcN
Description
PubMed
Regulation
constitutive expression at both protein and RNA levels PubMed
Expression
in Enterococcus faecalis: induced upon cell wall stress (vancomycin) (WhiA) PubMed
Regulatory mechanism
WhiA: transcription repression, described for Enterococcus faecalis PubMed, in whiA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
A ncRNA is predicted between 'yvcI' and 'TrxB' PubMed
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nahA→yvcN

2025-04-03 12:06:53

Jstuelk

154

BCC5699F6256B8A1B7FD8A979DBBBC9D4D1198AB

9244FB113E998572106D983A19AB3AC81B9F9D35

Additional information
translation is likely to require Efp due to the presence of several consecutive proline residues PubMed
Biological materials
Mutant
MGNA-B640 (yvcK::erm), available at the NBRP B. subtilis, Japan
BKE34760 (ΔglmR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCGATTTTCGGCTTTTGTC,  downstream forward: _UP4_TGAAGCCTTGAAATGAGGTG
BKK34760 (ΔglmR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCGATTTTCGGCTTTTGTC,  downstream forward: _UP4_TGAAGCCTTGAAATGAGGTG
Expression vectors
pGP736 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Boris Görke's lab
Labs working on this gene/protein
Anne Galinier, University of Marseille, France
References
Reviews
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Original Publications
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7150BABA5086D5E2EB8102E4901A216F43576282

Page visits: 7158

Time of last update: 2025-04-03 23:50:20

Author of last update: Jstuelk