GlnR

• Description: transcriptional repressor (MerR family) of the glnR-glnA operon
  
  

Gene name	glnR
Synonyms
Essential	no
Product	transcription repressor (MerR family)
Function	regulation of glutamine synthesis
Gene expression levels in SubtiExpress: glnR
Interactions involving this protein in SubtInteract: GlnR
Metabolic function and regulation of this protein in SubtiPathways: glnR
MW, pI	15 kDa, 9.731
Gene length, protein length	405 bp, 135 aa
Immediate neighbours	ynbB, glnA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, glutamate metabolism, transcription factors and their control

This gene is a member of the following regulons

GlnR regulon, TnrA regulon

The GlnR regulon:

• glnR-glnA, tnrA, ureA-ureB-ureC

The gene

Basic information

• Locus tag: BSU17450

Database entries

• BsubCyc: BSU17450

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: MerR family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): feedback-inhibited GlnA PubMed

• Effectors of protein activity: activity is enhanced upon interaction of the C-terminal domain with GlnA PubMed

• Interactions:
  • GlnR-GlnA, with feedback-inhibited GlnA, this results in DNA binding PubMed

• Localization:

Database entries

• BsubCyc: BSU17450

• Structure:
  • 4R4E (the GlnR-DNA complex) PubMed

• UniProt: P37582

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: glnR-glnA PubMed

• Expression browser: glnR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of glutamine (GlnR) PubMed
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism:
  • GlnR-GlnA complex: transcription repression PubMed
  • TnrA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: BP148 (del(glnR-glnA)::cat), available in Fabian Commichau's lab

• Expression vector:

• lacZ fusion: pGP189 (in pAC7), available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

References

Reviews

Katrin Gunka, Fabian M Commichau
Control of glutamate homeostasis in Bacillus subtilis: a complex interplay between ammonium assimilation, glutamate biosynthesis and degradation.
Mol Microbiol: 2012, 85(2);213-24
[PubMed:22625175] [WorldCat.org] [DOI] (I p)