yetO

yetO
168

cytochrome P450 (CYP102A2)/ NADPH-cytochrome P450 reductase

Locus
BSU_07250
Molecular weight
119.26 kDa
Isoelectric point
5.73
Protein length
1061 aaSequenceBlast
Gene length
3186 bpSequenceBlast
Function
fatty acid metabolism
Product
NADPH-cytochrome P450 reductase
Essential
no
E.C.
1.6.2.4
Synonyms
yetO, yfnJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
792,682  795,867
The protein
Catalyzed reaction/ biological activity
oxidation of even- and odd-chain saturated and unsaturated fatty acids to the respective omega-3, omega-2 and omega-1 hydroxylated fatty acids PubMed
alkane + O2 + reduced [NADPH—hemoprotein reductase] --> primary alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase] (according to UniProt)
NADPH + 2 oxidized [cytochrome P450] --> H+ + NADP+ + 2 reduced [cytochrome P450] (according to UniProt)
Protein family
cytochrome P450 family (according to UniProt)
Domains
Flavodoxin-like domain (aa 493-632) (according to UniProt)
FAD-binding FR-type domain (aa 671-904) (according to UniProt)
Cofactors
FAD (according to UniProt)
Structure
2X7Y (PDB) (from Bacillus megaterium; 66% identity, 87% similarity)  PubMed
O08394 (AlphaFold)
Paralogous protein(s)
YrhJ
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
yetO
Description
PubMed
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yetO

2025-03-29 16:07:44

Jstuelk

63

3464780681882c900eb43da61a692e9ac55909d5

6A780364E857EE722D7FBA2C73BB7BB800B08977

Biological materials
Mutant
MGNA-C225 (yfnJ::erm), available at the NBRP B. subtilis, Japan
BKE07250 (yetO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGAACCATTCCTTTCCG,  downstream forward: _UP4_TAGATAAAGAAGACTGGAGA
BKK07250 (yetO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGAACCATTCCTTTCCG,  downstream forward: _UP4_TAGATAAAGAAGACTGGAGA
References
Hyster TK, Farwell CC, Buller AR, McIntosh JA, Arnold FH Enzyme-controlled nitrogen-atom transfer enables regiodivergent C-H amination. Journal of the American Chemical Society. 2014 Nov 05; 136(44):15505-8. doi:10.1021/ja509308v. PMID:25325618
Kuper J, Tee KL, Wilmanns M, Roccatano D, Schwaneberg U, Wong TS The role of active-site Phe87 in modulating the organic co-solvent tolerance of cytochrome P450 BM3 monooxygenase. Acta crystallographica. Section F, Structural biology and crystallization communications. 2012 Sep 01; 68(Pt 9):1013-7. doi:10.1107/S1744309112031570. PMID:22949185
Axarli I, Prigipaki A, Labrou NE Cytochrome P450 102A2 Catalyzes Efficient Oxidation of Sodium Dodecyl Sulphate: A Molecular Tool for Remediation. Enzyme research. 2010 Jul 01; 2010:125429. doi:10.4061/2010/125429. PMID:21048857
Eiben S, Kaysser L, Maurer S, Kühnel K, Urlacher VB, Schmid RD Preparative use of isolated CYP102 monooxygenases -- a critical appraisal. Journal of biotechnology. 2006 Aug 05; 124(4):662-9. . PMID:16716428
Axarli I, Prigipaki A, Labrou NE Engineering the substrate specificity of cytochrome P450 CYP102A2 by directed evolution: production of an efficient enzyme for bioconversion of fine chemicals. Biomolecular engineering. 2005 Jun; 22(1-3):81-8. . PMID:15857787
Budde M, Maurer SC, Schmid RD, Urlacher VB Cloning, expression and characterisation of CYP102A2, a self-sufficient P450 monooxygenase from Bacillus subtilis. Applied microbiology and biotechnology. 2004 Dec; 66(2):180-6. . PMID:15375636
Gustafsson MC, Roitel O, Marshall KR, Noble MA, Chapman SK, Pessegueiro A, Fulco AJ, Cheesman MR, von Wachenfeldt C, Munro AW Expression, purification, and characterization of Bacillus subtilis cytochromes P450 CYP102A2 and CYP102A3: flavocytochrome homologues of P450 BM3 from Bacillus megaterium. Biochemistry. 2004 May 11; 43(18):5474-87. . PMID:15122913
Gustafsson MC, Palmer CN, Wolf CR, von Wachenfeldt C Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species. Archives of microbiology. 2001 Dec; 176(6):459-64. . PMID:11734890

9AA8CAEB42B1ED4CC8D80D57B045A55307114D6A

Page visits: 4345

Time of last update: 2025-04-10 01:28:22

Author of last update: Melvin.boenninger