yetO

yetO
168

cytochrome P450 (CYP102A2)/ NADPH-cytochrome P450 reductase

Locus
BSU_07250
Molecular weight
119.26 kDa
Isoelectric point
5.73
Protein length
1061 aaSequenceBlast
Gene length
3186 bpSequenceBlast
Function
fatty acid metabolism
Product
NADPH-cytochrome P450 reductase
Essential
no
E.C.
1.6.2.4
Synonyms
yetO, yfnJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
792,682  795,867
The protein
Catalyzed reaction/ biological activity
oxidation of even- and odd-chain saturated and unsaturated fatty acids to the respective omega-3, omega-2 and omega-1 hydroxylated fatty acids PubMed
alkane + O2 + reduced [NADPH—hemoprotein reductase] --> primary alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase] (according to UniProt)
NADPH + 2 oxidized [cytochrome P450] --> H+ + NADP+ + 2 reduced [cytochrome P450] (according to UniProt)
Protein family
cytochrome P450 family (according to UniProt)
Domains
Flavodoxin-like domain (aa 493-632) (according to UniProt)
FAD-binding FR-type domain (aa 671-904) (according to UniProt)
Cofactors
FAD (according to UniProt)
Structure
2X7Y (PDB) (from Bacillus megaterium; 66% identity, 87% similarity)  PubMed
O08394 (AlphaFold)
Paralogous protein(s)
YrhJ
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
yetO
Description
PubMed
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yetO

2025-03-29 16:07:44

Jstuelk

63

3464780681882c900eb43da61a692e9ac55909d5

6A780364E857EE722D7FBA2C73BB7BB800B08977

Biological materials
Mutant
MGNA-C225 (yfnJ::erm), available at the NBRP B. subtilis, Japan
BKE07250 (yetO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGAACCATTCCTTTCCG,  downstream forward: _UP4_TAGATAAAGAAGACTGGAGA
BKK07250 (yetO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGAACCATTCCTTTCCG,  downstream forward: _UP4_TAGATAAAGAAGACTGGAGA
References
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Page visits: 4347

Time of last update: 2025-04-10 02:33:32

Author of last update: Melvin.boenninger