Gmk

• Description: guanylate kinase (GMP:dATP, dGMP:ATP)
  
  

Gene name	gmk
Synonyms	yloD
Essential	yes PubMed
Product	guanylate kinase (GMP:dATP, dGMP:ATP)
Function	GTP biosynthesis
Gene expression levels in SubtiExpress: gmk
Metabolic function and regulation of this protein in SubtiPathways: gmk
MW, pI	23 kDa, 4.616
Gene length, protein length	612 bp, 204 aa
Immediate neighbours	ylzA, yloH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes, phosphoproteins

The gene

Basic information

• Locus tag: BSU15680

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15680

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + GMP = ADP + GDP (according to Swiss-Prot)

• Protein family: guanylate kinase-like domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-134 and/or Arg-149PubMed

• Cofactor(s):

• Effectors of protein activity:
  • inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´PubMed

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU15680

• Structure: 1S4Q (from Mycobacterium tuberculosis h37rv, 39% identity, 60% similarity)

• UniProt: O34328

• KEGG entry: [2]

• E.C. number: 2.7.4.8

Expression and regulation

• Operon:

• Expression browser: gmk PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 254 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1208 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1529 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 895 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1794 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kuanqing Liu, Angela R Myers, Tippapha Pisithkul, Kathy R Claas, Kenneth A Satyshur, Daniel Amador-Noguez, James L Keck, Jue D Wang
Molecular mechanism and evolution of guanylate kinase regulation by (p)ppGpp.
Mol Cell: 2015, 57(4);735-749
[PubMed:25661490] [WorldCat.org] [DOI] (I p)

Yuhta Nomura, Atsushi Izumi, Yoshinori Fukunaga, Kensuke Kusumi, Koh Iba, Seiya Watanabe, Yoichi Nakahira, Andreas P M Weber, Akira Nozawa, Yuzuru Tozawa
Diversity in guanosine 3',5'-bisdiphosphate (ppGpp) sensitivity among guanylate kinases of bacteria and plants.
J Biol Chem: 2014, 289(22);15631-41
[PubMed:24722991] [WorldCat.org] [DOI] (I p)

Alycia N Bittner, Allison Kriel, Jue D Wang
Lowering GTP level increases survival of amino acid starvation but slows growth rate for Bacillus subtilis cells lacking (p)ppGpp.
J Bacteriol: 2014, 196(11);2067-76
[PubMed:24682323] [WorldCat.org] [DOI] (I p)

Allison Kriel, Alycia N Bittner, Sok Ho Kim, Kuanqing Liu, Ashley K Tehranchi, Winnie Y Zou, Samantha Rendon, Rui Chen, Benjamin P Tu, Jue D Wang
Direct regulation of GTP homeostasis by (p)ppGpp: a critical component of viability and stress resistance.
Mol Cell: 2012, 48(2);231-41
[PubMed:22981860] [WorldCat.org] [DOI] (I p)

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)