phoP

phoP
168

two-component response regulator (OmpR family), regulation of phosphate metabolism

Locus
BSU_29110
Molecular weight
27.53 kDa
Isoelectric point
5.07
Protein length
240 aaSequenceBlast
Gene length
723 bpSequenceBlast
Function
regulation of phosphate metabolism (phoA, phoB, phoD, resABCDE, tagA-tagB, tagDEF, tuaA-H)
Product
two-component response regulator (OmpR family)
Essential
no
Synonyms
phoP
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0745 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,977,800  2,978,522
RNA
Interactions
phoS - 5' UTR of phoP PubMed
The protein
Protein family
OmpR family of two-component response regulators
Domains
Response regulatory domain (aa 4-118) (according to UniProt)
Structure
2OQR (PDB) (from Mycobacterium tuberculosis, 44% identity) PubMed
1MVO (PDB) (receiver domain)
P13792 (AlphaFold)
Modification
phosphorylation by PhoR under conditions of phosphate limitation (stimulates DNA-binding activity)
Effectors of protein activity
phosphorylation stimulates DNA-binding activity
Paralogous protein(s)
YkoG, YclJ, WalR
Localization
cytoplasm (according to UniProt)
Expression and Regulation
Operons
Genes
phoP-phoR
Description
PubMed
Regulation
carbon catabolite repression (CcpA) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
ScoC: repression, PubMed, in scoC regulon
PhoP: activation, due to PhoP~P binding to the 3’end of the PhoP-PhoR operon PubMed, in phoP regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigB: sigma factor, PubMed, in sigB regulon
SigE: sigma factor, PubMed, in sigE regulon
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phoP→phoR

2025-04-09 21:49:48

ghost

83

77b20114ede7ff98254c6266ed9893b85937a0ce

8B17CBE48E93642520F42C47E51A37FFB4AF27A8

Additional information
translation of phoP is limited by sequestration of the ribosomal binding site, the RBS is released upon binding of the phoS RNA PubMed
Biological materials
Mutant
1A966 ( phoP::tet), PubMed, available at BGSC
BKE29110 (phoP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGTGCCTCCAGTATT,  downstream forward: _UP4_TATAAACTGGAGGAGCCAAA
BKK29110 (phoP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGTGCCTCCAGTATT,  downstream forward: _UP4_TATAAACTGGAGGAGCCAAA
Labs working on this gene/protein
Marion Hulett, University of Illinois at Chicago, USA Homepage
References
Reviews
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Original Publications
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Targets of PhoR
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Biochemical analyses
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638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE

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Time of last update: 2025-04-09 23:00:08

Author of last update: Jstuelk