Difference between revisions of "YwlE"

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(References)
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**''ywlE::tet'' available from [[Ulf Gerth]]'s lab
 
**''ywlE::tet'' available from [[Ulf Gerth]]'s lab
 
**''ywlE::spec'' available from [[Ulf Gerth]]'s lab
 
**''ywlE::spec'' available from [[Ulf Gerth]]'s lab
**GP1458 (''ywlE''::''aphA3''), available in [[Jörg Stülke]]'s lab
+
** GP1458 (''ywlE''::''aphA3''), available in [[Jörg Stülke]]'s lab
**GP1459 (''ywlE''::''tet''), available in [[Jörg Stülke]]'s lab
+
** GP1459 (''ywlE''::''tet''), available in [[Jörg Stülke]]'s and [[Fabian Commichau]]'s labs {{PubMed|25610436}}
  
 
* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [[Ulf Gerth]]'s lab
 
* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [[Ulf Gerth]]'s lab
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 
* [[Ulf Gerth]], Greifswald, Germany
 
* [[Ulf Gerth]], Greifswald, Germany
 
 
* [[Fabian Commichau]] Göttingen, Germany
 
* [[Fabian Commichau]] Göttingen, Germany
  
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=References=
 
=References=
  
<pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742 23770242 23838530 24263382 24825175 </pubmed>
+
<pubmed>16452434,15866923,15995210,19678837 21622759, 20852588 22517742 23770242 23838530 24263382 24825175 25610436</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:06, 23 January 2015

Gene name ywlE
Synonyms ipc-31d
Essential no
Product protein arginine phosphatase
Function dephosphorylation of McsB
Gene expression levels in SubtiExpress: ywlE
MW, pI 16 kDa, 6.681
Gene length, protein length 450 bp, 150 aa
Immediate neighbours ywlF, ywlD
Sequences Protein DNA DNA_with_flanks
Genetic context
YwlE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YwlE expression.png















Categories containing this gene/protein

protein modification

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU36930

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Protein arginine phosphate + H2O = protein arginine + phosphate PubMed
  • Protein family:
  • Paralogous protein(s): YfkJ

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 91 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 272 PubMed

Biological materials

  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:

Labs working on this gene/protein

Your additional remarks

References