ypjD

ypjD
168

putative pyrophosphatase

Locus
BSU_22500
Molecular weight
12.83 kDa
Isoelectric point
4.95
Protein length
111 aaSequenceBlast
Gene length
336 bpSequenceBlast
Function
unknown
Product
putative pyrophosphatase
Essential
no
Synonyms
ypjD, jojD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1694 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,360,155  2,360,490
The protein
Structure
2GTA (PDB)
P42979 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
ypjD-dapB-mgsA-bshB1-bshA-cca-birA
Description
PubMed
Regulation
induced by diamide stess (thiol depletion) (Spx) PubMed
Regulatory mechanism
Spx: activation, PubMed, in spx regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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ypjD→birA

2025-03-13 06:13:52

ghost

92

53bf67b167bd77fe013a5e22a2f6ddcbf0c547e2

73042872A92944881DD9D94DF293F1857E4735FB

Biological materials
Mutant
BKE22500 (ypjD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGAACGAAACCTCCTAT,  downstream forward: _UP4_GAAGGAAAGTAGAGGAGATC
BKK22500 (ypjD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGAACGAAACCTCCTAT,  downstream forward: _UP4_GAAGGAAAGTAGAGGAGATC
LacZ fusion
pGP2437 (in pAC7), available in Jörg Stülke's lab
References
Operon and expression
Gaballa A, Antelmann H, Hamilton CJ, Helmann JD Regulation of Bacillus subtilis bacillithiol biosynthesis operons by Spx. Microbiology (Reading, England). 2013 Oct; 159(Pt 10):2025-35. doi:10.1099/mic.0.070482-0. PMID:23894131
Gaballa A, Newton GL, Antelmann H, Parsonage D, Upton H, Rawat M, Claiborne A, Fahey RC, Helmann JD Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli. Proceedings of the National Academy of Sciences of the United States of America. 2010 Apr 06; 107(14):6482-6. doi:10.1073/pnas.1000928107. PMID:20308541

A828298EA2A002538876FBA6113648AAD9C4C317

Page visits: 2597

Time of last update: 2025-04-06 09:43:37

Author of last update: Melvin.boenninger