dnaK

dnaK
168

class I heat-shock protein (molecular chaperone)

Locus
BSU_25470
Molecular weight
65.83 kDa
Isoelectric point
4.57
Protein length
611 aaSequenceBlast
Gene length
1836 bpSequenceBlast
Function
protein quality control
Product
class I heat-shock protein (molecular chaperone)
Essential
no
Synonyms
dnaK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0443 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,626,112 2,627,947
Phenotypes of a mutant
reduced protein secretion PubMed
a tig dnaK double mutant exhibits an aberrant twisted and filamentous cell morphology, as well as decreased tolerance to heat and to cell wall active antibiotics and hydrolytic enzymes, indicative of defects in cell wall integrity PubMed
The protein
Protein family
heat shock protein 70 family (single member, according to UniProt)
Structure
2V7Y (PDB) (from Geobacillus kaustophilus, 87% identity) PubMed
4ANI (PDB) (DnaK-GrpE complex from Geobacillus kaustophilus, 87% identity) PubMed
P17820 (AlphaFold)
Modification
phosphorylated on Tyr-601 by PtkA, dephosphorylated by PtpZ PubMed
Localization
cytoplasm (according to Swiss-Prot)
membrane-proximal (Spotty) PubMed
recruited to the membrane after ethanol stress PubMed
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV
Description
PubMed
Regulation
induced by heat shock (HrcA) PubMed
Regulatory mechanism
HrcA: repression, PubMed, in hrcA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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hrcA→yqeV

2025-04-11 08:16:44

ghost

166

5537cbdd2a5f5134768e8ba4f02922d86d6e912b

1FEFD4DF6108A61DA84FEA3CD39009FF4CA3EC3B

Biological materials
Mutant
BKE25470 (dnaK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTGAATAACCTCCTGCT, downstream forward: _UP4_TAAGTTCTTTTTAGTGTCAG
BKK25470 (dnaK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTGAATAACCTCCTGCT, downstream forward: _UP4_TAAGTTCTTTTTAGTGTCAG
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab
References
Reviews
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Original Publications
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30E0AAABB803E577D0D9FBAEF9031319CABD3D89

Page visits: 8035

Time of last update: 2025-04-16 15:32:35

Author of last update: Jstuelk