glxA

glxA
168

glyoxalase I

Locus
BSU_38370
Molecular weight
14.29 kDa
Isoelectric point
4.43
Protein length
126 aaSequenceBlast
Gene length
381 bpSequenceBlast
Function
detoxification of methylglyoxal
Product
glyoxalase I
Essential
no
Synonyms
glxA, ipa-18r, ywbC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0346 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,937,135  3,937,515
Phenotypes of a mutant
increased sensitivity to methylglyoxal PubMed
The protein
Catalyzed reaction/ biological activity
methylglyoxal + bacillithiol --> S-lactoyl-bacillithiol PubMed
Protein family
glyoxalase I family (with YraH and GlxB, according to UniProt)
Domains
VOC domain (aa 4-126) (according to UniProt)
Structure
1F9Z (PDB) (from E. coli, 35% identity) PubMed
P39586 (AlphaFold)
Expression and Regulation
Operons
Genes
glxA
Description
PubMed
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glxA

2025-02-15 13:25:55

ghost

78

6dcf5e474217964322e7c24acc7b0c7a92c16d76

53668B6BE2BE64AA0ADA0A18B8082794880E57BC

Biological materials
Mutant
MGNA-B221 (ywbC::erm), available at the NBRP B. subtilis, Japan
BKE38370 (glxA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGCTCCTCCTCGTAA,  downstream forward: _UP4_TAAAAATAAAGAACGTACAT
BKK38370 (glxA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTGCTCCTCCTCGTAA,  downstream forward: _UP4_TAAAAATAAAGAACGTACAT
GP4217 (glxA::kan), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
References
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Page visits: 3052

Time of last update: 2025-04-03 19:44:01

Author of last update: Jstuelk