sufC
168

ABC transporter (ATP-binding protein), synthesis of Fe-S clusters

Locus

BSU_32710

Molecular weight

28.88 kDa

Isoelectric point

4.62

Protein length

261 aaSequenceBlast

Gene length

786 bpSequenceBlast

Function

synthesis of Fe-S clusters

Product

ABC transporter (ATP-binding protein)

Essential

yes

Synonyms

sufC, yurY

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Cellular processes, Transporters, ABC transporters, Regulatory ABC transporters

Metabolism, Additional metabolic pathways, Iron metabolism, Biosynthesis of iron-sulfur clusters

Groups of genes, Essential genes

Groups of genes, Phosphoproteins, Phosphorylation on either a Ser, Thr or Tyr residue

List of homologs in different organisms, belongs to COG0396 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon

Gene

Coordinates

3,359,995 3,360,780

Phenotypes of a mutant

essential PubMed

the mutant is viable and Fe-S clusters are dispensable if IspG and IspH from the MEP pathway for isoprenoid biosynthesis are bypassed by a heterologous mevalonate pathway which allows B. subtilis to synthesize isopentenyl diphosphate and diphosphomevalonate PubMed

The protein

Catalyzed reaction/ biological activity

synthesis of Fe-S-clusters (the SufB-SufC-SufD complex) PubMed

Protein family

ABC transporter superfamily (according to UniProt)

Domains

ABC transporter domain (aa 6-248) (according to UniProt)

Structure

5AWF (PDB) (from E. coli, the SufB-SufC-SufD complex, 51% identity) PubMed

P80866 (AlphaFold)

Modification

phosphorylated on ser/ thr/ tyr PubMed

Additional information

belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

Genes

sufC-sufD-sufS-sufU-sufB

Description

PubMed

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

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Biological materials

Mutant

MGNA-B587 (yurY::erm), available at the NBRP B. subtilis, Japan

Two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab

References

Reviews

Dussouchaud M, Barras F, de Choudens SOFe-S biogenesis by SMS and SUF pathways: A focus on the assembly step.Biochimica et biophysica acta. Molecular cell research. 2024 Jun 3; 1871(7):119772. PMID: 38838856

Esquilin-Lebron K, Dubrac S, Barras F, Boyd JMBacterial Approaches for Assembling Iron-Sulfur Proteins.mBio. 2021 Dec 21; 12(6):e0242521. PMID: 34781750

Original Publications

O'Reilly FJ, Graziadei A, Forbrig C, Bremenkamp R, Charles K, Lenz S, Elfmann C, Fischer L, Stülke J, Rappsilber JProtein complexes in cells by AI-assisted structural proteomics.Molecular systems biology. 2023 Feb 23; :e11544. PMID: 36815589

Yokoyama N, Nonaka C, Ohashi Y, Shioda M, Terahata T, Chen W, Sakamoto K, Maruyama C, Saito T, Yuda E, Tanaka N, Fujishiro T, Kuzuyama T, Asai K, Takahashi Y Distinct roles for U-type proteins in iron-sulfur cluster biosynthesis revealed by genetic analysis of the Bacillus subtilis sufCDSUB operon. Molecular microbiology. 2018 Jan 02; . doi:10.1111/mmi.13907. PMID:29292548

Melvin RL, Salsbury FR Visualizing ensembles in structural biology. Journal of molecular graphics & modelling. 2016 Jun; 67:44-53. doi:10.1016/j.jmgm.2016.05.001. pii:S1093-3263(16)30073-0. PMID:27179343

Hirabayashi K, Yuda E, Tanaka N, Katayama S, Iwasaki K, Matsumoto T, Kurisu G, Outten FW, Fukuyama K, Takahashi Y, Wada K Functional Dynamics Revealed by the Structure of the SufBCD Complex, a Novel ATP-binding Cassette (ABC) Protein That Serves as a Scaffold for Iron-Sulfur Cluster Biogenesis. The Journal of biological chemistry. 2015 Dec 11; 290(50):29717-31. doi:10.1074/jbc.M115.680934. PMID:26472926

Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis. Science (New York, N.Y.). 2012 Mar 02; 335(6072):1103-6. doi:10.1126/science.1206848. PMID:22383849

Albrecht AG, Netz DJ, Miethke M, Pierik AJ, Burghaus O, Peuckert F, Lill R, Marahiel MA SufU is an essential iron-sulfur cluster scaffold protein in Bacillus subtilis. Journal of bacteriology. 2010 Mar; 192(6):1643-51. doi:10.1128/JB.01536-09. PMID:20097860

Lévine A, Vannier F, Absalon C, Kuhn L, Jackson P, Scrivener E, Labas V, Vinh J, Courtney P, Garin J, Séror SJ Analysis of the dynamic Bacillus subtilis Ser/Thr/Tyr phosphoproteome implicated in a wide variety of cellular processes. Proteomics. 2006 Apr; 6(7):2157-73. . PMID:16493705

Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M A comprehensive proteome map of growing Bacillus subtilis cells. Proteomics. 2004 Oct; 4(10):2849-76. . PMID:15378759

Quentin Y, Fichant G, Denizot F Inventory, assembly and analysis of Bacillus subtilis ABC transport systems. Journal of molecular biology. 1999 Apr 02; 287(3):467-84. . PMID:10092453

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Time of last update: 2025-04-04 12:03:46

Author of last update: Jstuelk

sufC 168

sufC
168