degS
168

two-component sensor kinase for exoenzyme and competence regulation, kinase activity is activated in response to inhibition of flagellar rotation

Locus

BSU_35500

Molecular weight

44.80 kDa

Isoelectric point

5.99

Protein length

385 aaSequenceBlast

Gene length

1158 bpSequenceBlast

Function

regulation of degradative enzyme and genetic competence

Product

two-component sensor kinase

Essential

Synonyms

degS, sacU

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4585 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

3,645,379 3,646,536

Phenotypes of a mutant

the mutation suppresses the mucoid phenotype of ''motA or motB'' mutants due to loss of DegU phosphorylation and concomitant reduced expression of the ''capB-capC-capA-capE'' operon PubMed

The protein

Catalyzed reaction/ biological activity

autophosphorylation, autodephosphorylation, phosphorylation of DegU on Asp-56 PubMed

ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

aa 12 ... 170: unknown DegS domain

Histidine kinase domain (aa 183-385) (according to UniProt)

aa 289 ... 384: HATPase_c

Structure

3GIG (PDB) (DesK, corresponds to the HisKA_3 and HATPase_c domains, 28% identity) PubMed

P13799 (AlphaFold)

Modification

phosphorylation on Ser-76 PubMed, this stimulates phosphotransfer from His-189 to DegU PubMed, the phosphorylyation is performed by PrkD in vitro PubMed

autophosphorylation on His-189

Effectors of protein activity

DegQ (stimulation of autophosphorylation) PubMed

inhibition of flagellar rotation stimulates kinase activity PubMed

Localization

Cytoplasm (Homogeneous) PubMed

Expression and Regulation

Operons

Genes

degS-degU

Description

PubMed