lgt

lgt
168

prolipoprotein diacylglyceryl transferase

Locus
BSU_34990
Molecular weight
30.47 kDa
Isoelectric point
8.96
Protein length
269 aaSequenceBlast
Gene length
810 bpSequenceBlast
Function
lipomodification of lipoproteins
Product
prolipoprotein diacylglyceryl transferase
Essential
no
E.C.
2.4.99.-
Synonyms
lgt, gerF, yvoC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0682 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,593,419  3,594,228
Phenotypes of a mutant
reduced ability of spores to germinate with alanine due ti reduced activity of the GerAA-GerAB-GerAC germinant receptor PubMed
inactivation of ''lgt'' reduces sporulation efficiency to 7.7% that of wild type cells PubMed
The protein
Catalyzed reaction/ biological activity
1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + L-cysteinyl-[prolipoprotein] --> H+ + S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[prolipoprotein] + sn-glycerol 1-phosphate (according to UniProt)
Protein family
lgt family (single member, according to UniProt)
Structure
5AZB (PDB) (from E. coli, 29% identity) PubMed
O34752 (AlphaFold)
Localization
membrane associated PubMed
Expression and Regulation
Operons
Genes
hprK-lgt-yvoD-yvoE-yvoF
Description
PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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hprK→yvoF

2025-03-30 14:12:07

ghost

116

93fddf316d690cb3235f223214494fee7a44414b

CC05676E9E34D6CA810B7283606A4417B30651B3

Biological materials
Mutant
MGNA-A388 (lgt::erm), available at the NBRP B. subtilis, Japan
GP851 (Δlgt-yvoD-yvoE-yvoF)::aphA3), available in Jörg Stülke's lab
1G22 ( lgt::erm), PubMed, available at BGSC
BKE34990 (Δlgt::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCTTCATTCATTTAACGCC,  downstream forward: _UP4_TAGACGTCAACAAAGGGGGA
BKK34990 (Δlgt::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCTTCATTCATTTAACGCC,  downstream forward: _UP4_TAGACGTCAACAAAGGGGGA
References
Reviews
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Original Publications
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Lgt in other organisms
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2EB790AD1872ABCB57B784296C04C773AEA9C22B

Page visits: 4565

Time of last update: 2025-04-02 09:00:30

Author of last update: Jstuelk