malK

malK
168

two-component sensor kinase, regulation of malate uptake

Locus
BSU_31520
Molecular weight
58.76 kDa
Isoelectric point
6.95
Protein length
533 aaSequenceBlast
Gene length
1602 bpSequenceBlast
Function
regulation of malate uptake
Product
two-component sensor kinase
Essential
no
E.C.
null
Synonyms
malK, yufL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3290 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,237,157  3,238,758
Phenotypes of a mutant
unable to use malate as the single carbon source PubMed
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of MalR
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
Domains
two transmembrane segments
PAS domain (aa 210-281) (according to UniProt)
Histidine kinase domain (aa 335-527) (according to UniProt)
Structure
O05250 (AlphaFold)
Modification
autophosphorylation on a His residue
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
malK-malR
Description
PubMed
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malK→malR

2025-04-04 12:18:52

Jstuelk

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4d0af3dc685749cd25b72d25b2068820a4e80caa

A1CE44BB5BE01A01460705F1FB2C87CE7994208A

Biological materials
Mutant
MGNA-A634 (yufL::erm), available at the NBRP B. subtilis, Japan
BKE31520 (malK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAAAACTTCCTTACGT,  downstream forward: _UP4_CCGTACGAACCGAAGGAGGA
BKK31520 (malK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAAAACTTCCTTACGT,  downstream forward: _UP4_CCGTACGAACCGAAGGAGGA
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
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Page visits: 3573

Time of last update: 2025-04-09 09:48:10

Author of last update: Melvin.boenninger