mutS

mutS
168

DNA mismatch repair (mismatch recognition protein)

Locus
BSU_17040
Molecular weight
97.39 kDa
Isoelectric point
5.19
Protein length
858 aaSequenceBlast
Gene length
2577 bpSequenceBlast
Function
DNA repair
Product
mismatch recognition protein
Essential
no
Synonyms
mutS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0249 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,775,745 → 1,778,321
Phenotypes of a mutant
60-fold increased mutation rate PubMed
a ''rnhB mutS-mutL'' mutant is not viable PubMed
The protein
Catalyzed reaction/ biological activity
MutS recognizes mismatches in the DNA and recruits MutL to the site of mismatch recognition
assists DNA strand exchange by facilitating RecA disassembly, and indirectly re-engagement with the homologous 5'-end of the linear duplex PubMed
modulates bidirectional RecA-mediated integration of divergent sequences PubMed
Protein family
DNA mismatch repair mutS family (with RqcU, according to UniProt)
Structure
1NG9 (PDB) (from E. coli, 39% identity) PubMed
P49849 (AlphaFold)
Localization
forms foci at midcell position, the frequency of foci increases upon mismatch formation PubMed
replication-active region of the DNA, via DnaN PubMed
MutS dynamics are heterogeneous in cells, with one MutS population exploring the nucleoid rapidly, while another MutS population moves to and transiently dwells at the replisome region, even in the absence of appreciable mismatch formation PubMed
Expression and Regulation
Operons
Genes
mutS-mutL
Description
PubMed
Additional information
A ncRNA is predicted between 'mutL' and 'ymzD' PubMed
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mutS→mutL

2025-04-08 19:10:48

ghost

106

904eecb53f1d0f2bcd0f561f80d88dc1faed6295

AD03305D7E5C23BD084F20F5C6F6EAC492F5C9FB

Biological materials
Mutant
GP1190 (ΔmutS-mutL::aphA3), available in Jörg Stülke's lab PubMed
1A833 ( mutS::spec), PubMed, available at Bacillus Genetic Stock Center
BKE17040 (ΔmutS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTTAATCCCTCACTA,  downstream forward: _UP4_TTACATTAAAACGGGGTGAT
BKK17040 (ΔmutS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTTTAATCCCTCACTA,  downstream forward: _UP4_TTACATTAAAACGGGGTGAT
References
Reviews
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Original Publications
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E2A8B04CD729332F9E6A13195181983F751F2F25

Page visits: 4067

Time of last update: 2025-04-10 09:02:21

Author of last update: Jstuelk