GreA

• Description: transcription elongation factor, resolves stalled RNA polymerase at promoter or promoter-proximal regions,
  
  

Gene name	greA
Synonyms
Essential	no
Product	transcription elongation factor
Function	transcription elongation
Gene expression levels in SubtiExpress: greA
MW, pI	17 kDa, 4.475
Gene length, protein length	471 bp, 157 aa
Immediate neighbours	pbpI, udk
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription

The gene

Basic information

• Locus tag: BSU27320

Phenotypes of a mutant

• RNA polymerase accumulates in promoter-proximal regions of many genes PubMed

Database entries

• BsubCyc: BSU27320

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: greA/greB family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GreA-RNA polymerase PubMed

• Localization: uniformly distributed throughout the nucleoid PubMed

Database entries

• BsubCyc: BSU27320

• Structure:

• UniProt: P80240

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: greA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 4395 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 11157 PubMed

Biological materials

• Mutant: GP351 (cm) available in Fabian Commichau's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Nikolay Zenkin
Multiple personalities of the RNA polymerase active centre.
Microbiology (Reading): 2014, 160(Pt 7);1316-1320
[PubMed:24763425] [WorldCat.org] [DOI] (I p)

Padraig Deighan, Ann Hochschild
Conformational toggle triggers a modulator of RNA polymerase activity.
Trends Biochem Sci: 2006, 31(8);424-6
[PubMed:16815708] [WorldCat.org] [DOI] (P p)

Sergei Borukhov, Jookyung Lee, Oleg Laptenko
Bacterial transcription elongation factors: new insights into molecular mechanism of action.
Mol Microbiol: 2005, 55(5);1315-24
[PubMed:15720542] [WorldCat.org] [DOI] (P p)

Bryce E Nickels, Ann Hochschild
Regulation of RNA polymerase through the secondary channel.
Cell: 2004, 118(3);281-4
[PubMed:15294154] [WorldCat.org] [DOI] (P p)

Original Publications

Alžbeta Rabatinová, Hana Šanderová, Jitka Jirát Matějčková, Jana Korelusová, Luděk Sojka, Ivan Barvík, Veronika Papoušková, Vladimír Sklenár, Lukáš Žídek, Libor Krásný
The δ subunit of RNA polymerase is required for rapid changes in gene expression and competitive fitness of the cell.
J Bacteriol: 2013, 195(11);2603-11
[PubMed:23543716] [WorldCat.org] [DOI] (I p)

Yoko Kusuya, Ken Kurokawa, Shu Ishikawa, Naotake Ogasawara, Taku Oshima
Transcription factor GreA contributes to resolving promoter-proximal pausing of RNA polymerase in Bacillus subtilis cells.
J Bacteriol: 2011, 193(12);3090-9
[PubMed:21515770] [WorldCat.org] [DOI] (I p)

Mohammad Roghanian, Yulia Yuzenkova, Nikolay Zenkin
Controlled interplay between trigger loop and Gre factor in the RNA polymerase active centre.
Nucleic Acids Res: 2011, 39(10);4352-9
[PubMed:21266474] [WorldCat.org] [DOI] (I p)

Geoff P Doherty, Donna H Meredith, Peter J Lewis
Subcellular partitioning of transcription factors in Bacillus subtilis.
J Bacteriol: 2006, 188(11);4101-10
[PubMed:16707701] [WorldCat.org] [DOI] (P p)