lytF

lytF
168

gamma-D-glutamate-meso-diaminopimelate muropeptidase, cell separase (minor autolysin)

Locus
BSU_09370
Molecular weight
51.23 kDa
Isoelectric point
10.41
Protein length
488 aaSequenceBlast
Gene length
1467 bpSequenceBlast
Function
cell separation
Product
gamma-D-glutamate-meso-diaminopimelate muropeptidase (minor autolysin)
Essential
no
E.C.
3.4.-.-
Synonyms
lytF, cwlE, yhdD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1388 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,011,792 → 1,013,258
Phenotypes of a mutant
cell separation defect, this is increased  by a ''lytE'' mutation PubMed
The protein
Catalyzed reaction/ biological activity
cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) PubMed
degradation of gamma-polyglutamic acid PubMed
Protein family
Peptidase C40 family (according to UniProt)
Domains
contains five N-acetylglucosamine-polymer-binding LysM domains PubMed
C-terminal D,L-endopeptidase domain (NlpC/P60 domain) PubMed
5 LysM domains (aa 27-70, aa 92-135, aa 174-217, aa 240-283, aa 307-350) (according to UniProt)
Structure
4XCM (PDB) (from Thermus thermophilus, 31% identity) PubMed
O07532 (AlphaFold)
Effectors of protein activity
both enzymatic activities are inhibited by interaction with IseA PubMed
Localization
secreted (according to Swiss-Prot)
localizes to cell septa and poles on the vegetative cell surface, this depends on the presence of lipoteichoic acids (LtaS) PubMed
for an analysis of motion of LytF, see PubMed
Expression and Regulation
Operons
Genes
lytF
Description
PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
SlrR: repression, in slrR regulon
SinR: repression, in sinR regulon
Sigma factors
SigD: sigma factor, PubMed, in sigD regulon
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lytF

2025-04-07 05:15:09

Jstuelk

95

93D37C770DBC29173ED55554AC405BE1CF2A4481

C2F505BFD930331384F17271E0CC90CA7B008F56

Biological materials
Mutant
1A791 ( lytF::spec), PubMed, available at BGSC
1A792 ( lytF::spec), PubMed, available at BGSC
BKE09370 (ΔlytF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTAGCTCTCCTTTTTC,  downstream forward: _UP4_TAAAAACAGAAACTGTGCGG
BKK09370 (ΔlytF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTAGCTCTCCTTTTTC,  downstream forward: _UP4_TAAAAACAGAAACTGTGCGG
References
Reviews
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Original Publications
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E8B88CBE4F9121DFEEF099D7947CD1E1AE656160

Page visits: 6983

Time of last update: 2025-04-23 04:08:04

Author of last update: Jstuelk