motI

motI
168

c-di-GMP receptor protein, inhibits flagellar rotation at high c-di-GMP concentrations

Locus
BSU_22910
Molecular weight
24.98 kDa
Isoelectric point
7.88
Protein length
217 aaSequenceBlast
Gene length
654 bpSequenceBlast
Function
control of MotA activity
Product
c-di-GMP receptor protein
Essential
no
Synonyms
motI, dgrA, ypfA, jofA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG5581 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,397,019 → 2,397,672
The protein
Domains
contains a C-terminal PilZ domain (aa 98-203) PubMed
Cofactors
c-di-GMP PubMed
Structure
5VX6 (PDB) (complex with c-di-GMP) PubMed
P38491 (AlphaFold)
Effectors of protein activity
binding of c-di-GMP stimulates the inhibitory interaction with MotA PubMed
Localization
forms MotA-dependent spots at the membrane PubMed
Expression and Regulation
Operons
Genes
motI-ypfB-cmk-rpsA-fni
Description
PubMed
Open in new tab

motI→fni

2025-03-17 18:30:36

Jstuelk

151

40589d593f2ed9acd667e06cee390577206bc679

B16C0E2FDCD0AB8461F12841F5F6E879CEC5BE2C

Genes
motI-ypfB-cmk-rpsA
Description
PubMed
Open in new tab

motI→rpsA

2025-03-17 13:51:16

Jstuelk

90

e8404d7a4ece2ad33ad4f2d223369df3e684ff6d

BB9E8E7CE0132FE9AD9D01FF7333E5A2FF968CEF

Biological materials
Mutant
MGNA-A419 (ypfA::erm), available at the NBRP B. subtilis, Japan
BKE22910 (ΔmotI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGACTTCACTCTTTCGC,  downstream forward: _UP4_TAAACATCGGCAAACACTTC
BKK22910 (ΔmotI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGACTTCACTCTTTCGC,  downstream forward: _UP4_TAAACATCGGCAAACACTTC
References
Reviews
Subramanian S, Kearns DB Functional Regulators of Bacterial Flagella. Annual review of microbiology. 2019 May 28; . doi:10.1146/annurev-micro-020518-115725. PMID:31136265
Mukherjee S, Kearns DB The structure and regulation of flagella in Bacillus subtilis. Annual review of genetics. 2014; 48:319-40. doi:10.1146/annurev-genet-120213-092406. PMID:25251856
Original Publications
Kunz S, Tribensky A, Steinchen W, Oviedo-Bocanegra L, Bedrunka P, Graumann PL Cyclic di-GMP Signaling in Bacillus subtilis Is Governed by Direct Interactions of Diguanylate Cyclases and Cognate Receptors. mBio. 2020 Mar 10; 11(2). pii:e03122-19. doi:10.1128/mBio.03122-19. PMID:32156823
Subramanian S, Gao X, Dann CE, Kearns DB MotI (DgrA) acts as a molecular clutch on the flagellar stator protein MotA in Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America. 2017 Dec 01; . pii:201716231. doi:10.1073/pnas.1716231114. PMID:29196522
Gao X, Mukherjee S, Matthews PM, Hammad LA, Kearns DB, Dann CE Functional characterization of core components of the Bacillus subtilis cyclic-di-GMP signaling pathway. Journal of bacteriology. 2013 Nov; 195(21):4782-92. doi:10.1128/JB.00373-13. PMID:23893111
Chen Y, Chai Y, Guo JH, Losick R Evidence for cyclic Di-GMP-mediated signaling in Bacillus subtilis. Journal of bacteriology. 2012 Sep; 194(18):5080-90. doi:10.1128/JB.01092-12. PMID:22821967

C194336ED96FA844FFE9FB43BE59ADC5D6DDB3BE

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Time of last update: 2025-04-10 05:20:44

Author of last update: Christoph.elfmann