csrA

csrA
168

motility regulator, binds to the hag mRNA to inhibit its translation, promotes the base pairing between the sr1 RNA and the ahrC mRNA

Locus
BSU_35370
Molecular weight
8.00 kDa
Isoelectric point
6.09
Protein length
74 aaSequenceBlast
Gene length
225 bpSequenceBlast
Function
control of hag translation
Product
RNA chaperone, motility regulator
Essential
no
Synonyms
csrA, yviG
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1551 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,636,046  3,636,270
The protein
Catalyzed reaction/ biological activity
CsrA binds the hag mRNA to inhibit translation PubMed
binds sr1 RNA and ahrC mRNA to promote their base pairing and to control arginine metabolism PubMed
Protein family
CsrA/RsmA family (single member, according to UniProt)
Domains
Contacts FliW via its C-terminus (residues 49-58) PubMed
Structure
1T3O (PDB)
5DMB (PDB) (FliW-CsrA complex) PubMed
P33911 (AlphaFold)
Effectors of protein activity
CsrA is sequestered by interaction with FliW (the two proteins form a conserved module in many bacteria) PubMed
Localization
cytoplasm (according to UniProt)
Additional information
CsrA and FliW form a conserved module in many bacteria PubMed
Expression and Regulation
Operons
Genes
comFA-comFB-comFC-yvyF-flgM-flgN-flgK-flgL-yviE-fliW-csrA
Description
PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
ComK: activation, PubMed, in comK regulon
DegU: activation, DegU-P, PubMed, in degU regulon
ScoC: repression, PubMed, in scoC regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

comFA→csrA

2025-03-28 01:21:10

Jstuelk

156

F13A7084BCCB15345BC26C03AF15EE753EFDF196

11180672E46D33F7D747E58441ECB10AEE7F8D8B

Genes
csrA
Description
PubMed
Sigma factors
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

csrA

2025-04-04 19:09:33

ghost

95

2796c2c2462afed153fcc96979a674f024f06bf1

DC6D0A8DC5F49EFA2C53BD3E355332E6C5560A6D

Biological materials
Mutant
MGNA-A392 (csrA::erm), available at the NBRP B. subtilis, Japan
GP469 (spc), available in Jörg Stülke's lab
BKE35370 (csrA::erm trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCGTTTATTTTCCGCGATA,  downstream forward: _UP4_TGAGGATTTTTTTATTTTTG
BKK35370 (csrA::kan trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCGTTTATTTTCCGCGATA,  downstream forward: _UP4_TGAGGATTTTTTTATTTTTG
Expression vectors
pGP381 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Jörg Stülke's lab
for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP383, available in Jörg Stülke's lab
LacZ fusion
pGP461 (in pAC7), available in Jörg Stülke's lab
References
Reviews
Loading
Original Publications
Loading

93F328524989597C7D2329B25E665496C9631E87

Page visits: 5612

Time of last update: 2025-04-05 04:44:56

Author of last update: Jstuelk