alaP

alaP
168

alanine permease (L-Ala, D-Ala, ß-Ala)

Locus
BSU_30530
Molecular weight
50.16 kDa
Isoelectric point
9.91
Protein length
463 aaSequenceBlast
Gene length
1392 bpSequenceBlast
Function
uptake of D- and L-alanine and of ß-alanine
Product
alanine permease
Essential
no
Synonyms
ytnA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1113 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,124,250  3,125,641
Phenotypes of a mutant
a alaP alr double mutant is not viable due to the inability to acquire D-alanine PubMed
The protein
Catalyzed reaction/ biological activity
uptake of D- and L-alanine and of ß-alanine PubMed
Protein family
amino acid-polyamine-organocation (APC) superfamily (according to UniProt)
Structure
6F34 (PDB) (from Geobacillus kaustophilus, 21% identity) PubMed
O34618 (AlphaFold)
Paralogous protein(s)
HutM, YvbW, AimB, RocC, RocE, YbgF, YdgF, AapA, GabP
Localization
Cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
metK-asnB-alaP
Description
PubMed
Regulation
constitutive
Open in new tab

metK→alaP

2025-04-03 13:31:47

Jstuelk

131

EBD1A656EE2CBA59E12ED2624758B322F0A2AF06

074501579DF980959E9B85A1859566DB48AC89A9

Biological materials
Mutant
GP3580 (ΔalaP::spec), available in Jörg Stülke's lab
GP4146 (ΔalaP::neo), available in Jörg Stülke's lab PubMed
MGNA-A126 (ytnA::erm), available at the NBRP B. subtilis, Japan
GP1885 (ΔalaP::spc) available in Jörg Stülke's lab PubMed
BKE30530 (alaP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTTCTTCTCCCCTAGA,  downstream forward: _UP4_TGACAAAAAGAGCTCCCAGT
BKK30530 (alaP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTTCTTCTCCCCTAGA,  downstream forward: _UP4_TGACAAAAAGAGCTCCCAGT
LacZ fusion
pGP3820 (in pAC7), available in Jörg Stülke's lab
References
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219586A3F378DC38EA076FD39B99D41136BDE722

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Time of last update: 2025-04-06 05:31:53

Author of last update: Jstuelk