IlvH

• Description: acetolactate synthase (small subunit)
  
  

Gene name	ilvH
Synonyms	ilvN
Essential	no
Product	acetolactate synthase (small subunit)
Function	biosynthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: ilvH
Metabolic function and regulation of this protein in SubtiPathways: ilvH
MW, pI	19 kDa, 10.295
Gene length, protein length	522 bp, 174 aa
Immediate neighbours	ilvC, ilvB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CcpA regulon, CodY regulon, T-box, TnrA regulon

The gene

Basic information

• Locus tag: BSU28300

Database entries

• BsubCyc: BSU28300

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO₂ (according to Swiss-Prot)

• Protein family: acetolactate synthase small subunit family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU28300

• Structure: 2F1F (regulatory subunit from Escherichia coli, 37% identity, 62% similarity) PubMed

• UniProt: P37252

• KEGG entry: [3]

• E.C. number: 2.2.1.6

Expression and regulation

• Operon: ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD PubMed

• Expression browser: ilvH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
  • repressed by casamino acids PubMed
  • expression is stimulated in the presence of glucose PubMed
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • glucose regulation: CcpA PubMed
  • repression by BCAA: tRNA-controlled RNA switch (T-box) that mediates termination/antitermination
  • TnrA: transcription repression PubMed
  • CodY: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 603 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: